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Anti rnf168 sc 101125

Manufactured by Santa Cruz Biotechnology
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Sourced in China

Anti‐RNF168 (SC‐101125) is a mouse monoclonal antibody that detects RNF168 protein. RNF168 is an E3 ubiquitin‐protein ligase involved in the cellular response to DNA double‐strand breaks. This antibody can be used for Western blot and immunohistochemical applications.

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Lab products found in correlation

Anti actin 8h10d10, by Cell Signaling Technology (2 mentions) Anti h3k27ac d5e4, by Cell Signaling Technology (1 mentions) Anti tubulin t5168, by Merck Group (1 mentions) Anti stat1 9172, by Cell Signaling Technology (1 mentions)

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Anti-RNF168 (2 citations) RNF168 (2 citations)

2 protocols using anti rnf168 sc 101125

1

Comprehensive Protein Expression Analysis

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Cells were lysed with RIPA lysis buffer. Anti‐ERɑ mouse (1D5, SC56833) was from Santa Cruz Biotechnology. Anti‐ERɑ rabbit (D8H8, #8644) was from Cell Signaling Technology. Anti‐RNF168 (SC‐101125) was acquired from Santa Cruz Biotechnology. Anti‐SRC1 (128E7), anti‐SRC3 (5E11) and anti‐H3K27ac (D5E4) antibodies were acquired from Cell Signaling Technology. Anti‐PolII (PLA0127) and anti‐P300 (HPA003128) were acquired from Sigma. Anti‐tubulin (T‐5168) and anti‐histone‐3 (Ab18521) were acquired from Sigma and Abcam, respectively. Anti‐actin (8H10D10) was acquired from Cell Signaling Technology.
Liu Z., Zhang J., Xu J., Yang H., Li X., Hou Y., Zhao Y., Xue M., Wang B., Yu N., Yu S., Niu G., Wu G., Li X., Wang H., Zhu J, & Zhuang T. (2018). RNF168 facilitates oestrogen receptor ɑ transcription and drives breast cancer proliferation. Journal of Cellular and Molecular Medicine, 22(9), 4161-4170.
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2

Protein Extraction and Immunoblotting

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RIPA lysis buffer was used to extract protein from cell lysis. Anti‐Tubulin mouse (T5168) was acquired from Sigma (Wuxi City, China). Anti‐actin (8H10D10) was acquired from Cell Signaling Technology (Beijing, China). Anti‐RNF168 (SC‐101125) was from Santa Cruz Biotechnology (Shanghai, China). Anti‐STAT1 (9172) was acquired from Cell Signaling Technology. anti‐Lamin B1 (66095‐1) was acquired from Wuhan Sanyin company (Wuhan, China).
Yu N., Xue M., Wang W., Xia D., Li Y., Zhou X., Pang D., Lu K., Hou J., Zhang A., Zhuang T., Wang L., Chang T, & Li X. (2018). RNF168 facilitates proliferation and invasion of esophageal carcinoma, possibly via stabilizing STAT1. Journal of Cellular and Molecular Medicine, 23(2), 1553-1561.
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