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3 protocols using mbaam

1

Lignin-Based Hydrogel Synthesis

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Mixed hardwood kraft lignin (LGN) was supplied
by FPInnovations’ pilot plant facility located in Thunder Bay,
ON. NIPAAm (97%), MBAAm (99%), azobisisobutyronitrile (AIBN, 98%),
acetone (97%), dimethyl sulfoxide-d6 (DMSO,
99.9% atom D), and tetramethylthionine chloride (methylene blue) were
obtained from Sigma-Aldrich. Sulfuric acid (98%) and sodium hydroxide
(97%) were also obtained from Sigma-Aldrich and diluted with deionized
water to 20 and 10%, respectively.
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2

Hydrogel-Encapsulated Chickpea Embryos

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(3-Acrylamidopropyl)-trimethylammonium chloride
(AMPTMA, 75 wt % in H2O, Sigma-Aldrich); methylene bis-acrylamide
(MBAAm, Sigma-Aldrich); poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide),
MW = 12,600 g·mol–1; the so-called Pluronic
F-127, Sigma-Aldrich; ammonium persulfate (APS, Fluka); N,N,N′,N′-tetramethylethylenediamine (TMEDA, Sigma-Aldrich); ethanol (Sigma-Aldrich);
diethyl ether (Merck); and toluene (Merck) were all used as received.
Embryo axes of chickpea (Cicer arietinum L.) were used as initial explants (see the Supporting Information).
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3

Synthesis of Photocatalytic Hydrogels

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Silver nitrate, ferric nitrate nonahydrate, polyvinylpyrrolidone, potassium hydroxide, ethanol, AAm, AA, MBAAm, BACM, ammonium persulphate, and methyl orange (MO) were purchased from Sigma Aldrich Trading Co., Ltd. (Shanghai, China). All reagents were used directly without further purification. Monocrystalline silicon was purchased from GRINM Semiconductor Materials Co., Ltd. (Beijing, China).
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