All animal protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Dartmouth College before the start of the study. Animals were maintained at the Dartmouth Animal Resource Center in a clean 12-h light/dark cycle room with free access to food and water. Four adult Fisher rats (Charles River Laboratories, MA, USA) were used for the following imaging procedures: ultrasound, micro-CT, clinical CT, clinical cone-beam CB-CT with OxyChip-GNP and clinically used fiducials. Adult female Balb/c mice (20–30g, Charles River Laboratories, MA, USA) were used to assess short term implantation effects of OxyChip-GNP. Two adult female Balb/c (20–30g, Charles River Laboratories, MA, USA) mice were used for muscle EPR pO2 tissue measurement with OxyChip and OxyChip-GNP and to assess for long-term (10 weeks) implantation effects of the OxyChip-GNP.
Adult female balb c mice
Manufactured by Charles River Laboratories
Sourced in United States
Adult female BALB/c mice are a widely used laboratory mouse strain. They are commonly used in various research applications to study immunology, infectious diseases, and cancer. The core function of these mice is to serve as a model organism for scientific investigation.
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5 protocols using adult female balb c mice
1
Multimodal Imaging and Implantation of OxyChip
2
BALB/c Mice Sourcing and Husbandry
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Adult female BALB/c mice were purchased from Charles River Canada (Saint-Constant, QC) and cared for in our institutional barrier facility.
3
Balb/c Mice Study Guidelines
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Adult female BALB/c mice (6–8 weeks) were obtained from Charles River (Sulzfeld, Germany). They were housed in groups in plastic cages with adsorbent bedding in a temperature and light-dark cycle (12 h:12 h) controlled room. Food and water were available ad libitum. All animal procedures were performed in an experimental animal laboratory; all surgical techniques were performed under sodium pentobarbital anaesthesia to minimise animal suffering. If any animals showed signs of ill health prior to the experimental endpoint (according to guidelines issued by Karolinska Institutet), the animals were sacrificed by cervical dislocation. At the end of each experimental time point, animals were sacrificed by cervical dislocation. All animal procedures were approved by the local ethics committee at Karolinska Institutet (Stockholm norra djurförsöksetiska nämnd; ethical permit numbers: 152/06 and 153/11).
4
Ovariectomized Female BALB/c Mice Protocol
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Adult female BALB/c mice (>8 wks; Charles River Laboratories, Wilmington, MA, USA) were used in the experiments described herein. All mice were allowed to acclimate to our facility for 1 wk after arrival, and then were ovariectomized to eliminate the covariate of acute ovarian failure in response to chemotherapy17 (link)18 (link). All surgeries were conducted using aseptic technique by an experienced surgeon (NZ), and mice were supplied analgesia (0.05 mg/kg buprenorphine, SC) and post-operative warmth (cage placement on a heating pad) until mobile. Ibuprofen (~30 mg/kg) was supplied in the drinking water for 5 days following surgery for further analgesia. Mice were group housed (4/cage) for 2 wks prior to experimental assignment. Mice were supplied ad libitum food (Harlan Teklad #7912) and filtered tap water, as well as a cotton nestlet. Cages were changed weekly. Mice were maintained on a 12:12 light/dark cycle. Body mass measures were obtained on the day prior to injection, and then at tissue collection 1, 3, 9, and 24 h post-injection. All experiments were approved and carried out in accordance with guidelines set by The Ohio State University Institutional Animal Care and Use Committee (IACUC).
5
Murine Model for Biomedical Research
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Adult female BALB/c mice (20 g) and adult female Wistar rats (200 g) were purchased from the Charles River Laboratories, Beijing, China. All the animals were housed at an individual plastic cage in a sterilized room with the temperature of 23.6 °C and humidity of 35% and a 12 h light/dark cycles (light on at 7:00 am). Each animal was used only once and fed sterile water and chow. All the animal experiments were in ABSL-2 laboratory and have been adopted by the Ethical Committee of Capital Medical University in China.
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