Prior to scarifying the mice, ectopic bone formation in the body was monitored by using a two-dimensional Soft X-ray apparatus (Hitex Ltd, Osaka, Japan) and a diagnostic X-ray film (X-OMAT V, Kodak, Rochester, NY, USA) under the following conditions; 35 kVp and 400 μA for 45 s. For three-dimensional analysis, each isolated specimen was scanned by micro-computed tomography (μ-CT; Skyscan 1172, Skyscan, Aartselaar, Belgium) in cone-beam acquisition mode. The X-ray source was set at 50 kV and 200 μA with a 0.5-mm aluminum filter at 17.09 μm resolution. The exposure time was 1.2 s and 257 projections were acquired over an angular range of 180° (angular step; 0.7°). The image slices were reconstructed by using the Nrecon program (version 1.6.2.0, Skyscan, Aartselaar, Belgium) and bone volume was measured using the CT-Analyzer program (version 1.10.0.5, Skyscan, Aartselaar, Belgium). Three dimensional surface rendering images were made using the Mimics software version 14.0, imaging program (Materialise N.V, Leuven, Belgium).
X omat 5
Manufactured by Kodak
Sourced in United States
The X-OMAT V is an automated film processor designed for the development of x-ray, medical, and industrial films. It features a microprocessor-controlled system that regulates the temperature, replenishment, and timing of the film processing cycle. The X-OMAT V is capable of handling a variety of film sizes and types, ensuring consistent and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using x omat 5
1
Ectopic Bone Formation Monitoring
2
Radiographic Analysis of Mouse Skeletal Morphology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The whole body and the isolated crania from each mouse were radiographed by 2-dimensional radiographic apparatus (Hitex Ltd., Osaka, Japan) using diagnostic X-ray film (X-OMAT V, Kodak, Rochester, NY, USA) under the following conditions 35 kVp and 400 μA for 45 s. For a 3-dimensional analysis, each specimen was scanned by micro-CT (Skyscan 1172; Skyscan, Aartselaar, Belgium) in cone-beam acquisition mode. The X-ray source was set at 50 kV and 200 μA with a 0.5 mm aluminum filter at 17.09 μm resolution. The exposure time was 1.2 s. 449 projections were acquired over an angular range of 180° (angular step; 0.4°). The image slices were reconstructed by using the NRecon program (version 1.6.2.0, Skyscan, Aartselaar, Belgium) and bone volume and thickness were measured using the CT-Analyzer program (version 1.10.0.5, Skyscan, Aartselaar, Belgium). 3D surface rendering images were obtained by using the Mimics software 14.0 (Materialise NV, Leuven, Belgium).
3
Radiographic Monitoring of Bone Regeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After capturing using a microscope, we used a Faxitron specimen radiography system (MX-20; Faxitron X-ray Co., Tucson, AZ) to monitor the treated area for the presence of the new bone at 3 and 4 weeks after scaffold implantation and cranial defect creation. Every skull sample was fixed using 10% buffered neutral formalin for 24 hours. After the fixation, samples were harvested using 75% alcohol. We then radiographed each sample using a diagnostic x-ray film (X-OMAT V; Kodak, Rochester, NY). Radiographs were scanned at 1200 dpi using the Microtek ScanMaker 9800XL (Microtek, Carson, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!