The largest database of trusted experimental protocols

4 protocols using anhydrous glucose

1

Evaluation of Antioxidant and Enzyme Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols
p-Nitrophenyl palmitate, p-nitrophenyl α-d-glucopyranoside (p-NPG), pancreatic lipase, orlistat, acarbose and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were all purchased from Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China. α-Glucosidase, gallic acid, rutin, ginsenoside Re, vitamin C and anhydrous glucose were obtained from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China. Folin–Ciocalteu reagent was purchased from Sangon Bioengineering (Shanghai) Co., Ltd., Shanghai, China. The total antioxidant (FRAP) kit was purchased from Nanjing Jiancheng Biological Engineering Research Institute Co., Ltd., Nanjing, China. The rest of the chemicals, reagents, consumables and culture media were purchased from National Pharmaceutical Chemical Reagent Co., Ltd., Shanghai, China.
+ Open protocol
+ Expand
2

Antioxidant Capacity Assays Using DPPH and FRAP

Check if the same lab product or an alternative is used in the 5 most similar protocols
1, 1-Diphenyl-2-trinitrophenylhydrazine (DPPH) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. Gallic acid, rutin, ginsenoside Re and anhydrous glucose were obtained from Shanghai Yuanye Biotechnology Co., Ltd, China. Folin-Ciocalteu phenol reagent was purchased from Sangon Bioengineering (Shanghai) Co., Ltd, China. The total antioxidant (FRAP) kit was purchased from Nanjing Jiancheng Biological Engineering Research Institute Co., Ltd, China. The rest of the chemicals, reagents, consumables and culture media were purchased from National Pharmaceutical Chemical Reagent Co., Ltd, China.
+ Open protocol
+ Expand
3

Analytical-grade reagents protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Analytical-grade reagents were purchased for this study: p-nitrophenyl-α-d-glucopyranoside (PNPG), tocopherol, hydroxybiphenyl, α-glucosidase (14.5 U/mg), acarbose, PBS (Sigma, Shanghai, China), ethanol, petroleum ether, methanol, DMSO, methanol (HPLC grade), 98% sulfuric acid, anthrone, sodium chloride, phosphate buffer, sodium carbonate, and sodium hydroxide (Nanjing Chemical Reagent Co., Ltd., China). AB-8 macroporous adsorption resin was purchased from Bengbu Liaoyuan New Materials Co., Ltd. Anhydrous glucose, DEAE cellulose, and dialysis bags were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Pectinase was purchased from Kandin Chemical Reagent Co., Ltd. (Guangzhou, China). DMEM was purchased from Thermo Fisher Scientific Instrument Co., Ltd. (Waltham, MA, USA).
+ Open protocol
+ Expand
4

Profiling Tea Polyphenols and Amino Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols
(‐)‐Catechin (C), (‐)‐epigallocatechin (EGC), (‐)‐gallocatechin (GC), (‐)‐epicatechin (EC), (‐)‐epicatechin gallate (ECG), (‐)‐epigallocatechin gallate (EGCG), (‐)‐gallocatechin gallate (GCG), anhydrous glucose, caffeine (CAF), and gallic acid (GA) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Company (Shanghai, China). Alanine (ala), arginine (arg), aspartic acid (asp), cysteine (cys), glutamic acid (glu), glycine (gly), histidine (his), isoleucine (ile), leucine (leu), methionine (met), phenylalanine (phe), proline (pro), serine (ser), threonine (thr), tryptophan (trp), tyrosine (tyr), valine (val), theanine, the AccQ‐Fluor Reagent Kit, and AccQ‐Tag eluent were purchased from BDH Chemicals Ltd. (Poole, UK). Acetonitrile (chromatographically pure), sodium carbonate, methanol (chromatographically pure), concentrated sulfuric acid, anthrone, ferrous tartrate (analytically pure), disodium hydrogen phosphate, and potassium dihydrogen phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Pure water from China Resources C'estbon Beverage Co., Ltd. (Shenzhen, China) was used throughout the study.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!