Losartan, lisinopril and AngII were purchased from Wako Pure Chemical (Tokyo, Japan). Benazepril was purchase from Tokyo Chemical Industry (Tokyo, Japan). PD123319, ZD7155, A779, SR202 and EHT1864 were purchased from Tocris Bioscience (Bristol, UK). Diphenylene iodium (DPI) and 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF) were purchased from Sigma-Aldrich. A water-soluble derivative of forskolin, 7-deacetyl-7-[O-(N-methylpiperazino)-γ-butyryl]-forskolin (forskolin-ws), was purchased from Calbiochem (San Diego, CA, USA).
Antagonists of AT1R (losartan and ZD7155) and AT2R (PD123319), the Mas receptor (A779) and PPARγ (SR202) and inhibitors of ACE (benazepril and lisinopril), NOX (DPI and AEBSF) and Rac (EHT1864) were dissolved in the perfused solution and administered to the striatum through the probe during the experimental period. Forskolin-ws, which stimulates cAMP production by activating adenylyl cyclase48 (link), and AngII were dissolved in sterilized physiological saline (Otsuka Pharmaceutical, Tokyo, Japan) and directly administered into the striatum through the thin needle of the MI-A-I-8-03 probe using an ESP-32 pump. The flow rate was 0.1 μL/min, and the total volume was 1 μL for forskolin-ws and 2 μL for AngII.
Antagonists of AT1R (losartan and ZD7155) and AT2R (PD123319), the Mas receptor (A779) and PPARγ (SR202) and inhibitors of ACE (benazepril and lisinopril), NOX (DPI and AEBSF) and Rac (EHT1864) were dissolved in the perfused solution and administered to the striatum through the probe during the experimental period. Forskolin-ws, which stimulates cAMP production by activating adenylyl cyclase48 (link), and AngII were dissolved in sterilized physiological saline (Otsuka Pharmaceutical, Tokyo, Japan) and directly administered into the striatum through the thin needle of the MI-A-I-8-03 probe using an ESP-32 pump. The flow rate was 0.1 μL/min, and the total volume was 1 μL for forskolin-ws and 2 μL for AngII.