U2OS cells were transfected with siScramble, siMMSET pool or siLigase IV. 24 h after transfection, cells were transfected with BamHI-XhoI linearized pEGFP-C1 (Clontech, Mountain View, CA, USA). The next day, cells were trypsinized, counted and plated. Cells with and without G418 (0.5 mg/ml) were incubated for 14 days at 37°C and colonies visualized with 0.05% crystal violet, 1% formaldehyde, 1% methanol. Quantification was performed using ImageJ.
Bamhi xhoi linearized pegfp c1
Manufactured by Takara Bio
Sourced in United States
BamHI-XhoI linearized pEGFP-C1 is a laboratory reagent used for gene expression studies. The plasmid is linearized with the restriction enzymes BamHI and XhoI, facilitating its use in various molecular biology techniques.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using bamhi xhoi linearized pegfp c1
1
Transfection-based colony formation assay
2
Transfection-based colony formation assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
U2OS cells were transfected with siScramble, siMMSET pool or siLigase IV. 24 h after transfection, cells were transfected with BamHI-XhoI linearized pEGFP-C1 (Clontech, Mountain View, CA, USA). The next day, cells were trypsinized, counted and plated. Cells with and without G418 (0.5 mg/ml) were incubated for 14 days at 37°C and colonies visualized with 0.05% crystal violet, 1% formaldehyde, 1% methanol. Quantification was performed using ImageJ.
3
Colony Formation Assay with HDGFRP3 KO
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Assays were performed as previously described (47 (link)) with minor modifications. Briefly, MDA-MB-231 and its derivative HDGFRP3 KO cells were transfected with BamHI-XhoI linearized pEGFP-C1 (Clontech). The following day, transfected cells were trypsinized and seeded in 10 cm dishes at different densities for colony formation. Selection was initiated the following day with 0.5 mg/ml G418. The cells on a plate lacking G418 were fixed to assess transfection efficiency. Colonies were then stained and counted. Random-plasmid integration events were normalized to transfection and plating efficiencies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!