Butyrylcholinesterase buche

Manufactured by Merck Group

Sourced in United States

Butyrylcholinesterase (BuChE) is a lab equipment product that functions as an enzyme responsible for the hydrolysis of the neurotransmitter acetylcholine. It plays a role in the regulation of cholinergic neurotransmission.

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Lab products found in correlation

Acetylcholinesterase, by Merck Group (1 mentions) Galantamine hydrobromide, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions) Uv 1700, by Shimadzu (1 mentions)

2 protocols using butyrylcholinesterase buche

Cholinesterase Enzyme Assay Protocol

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Acetylcholinesterase (AChE) from electric eel (C3389-2KU), butyrylcholinesterase (BuChE) from horse serum (E.C.3.1.1.8, Sigma St. Louis, MO, USA), acetylthiocholine iodide and butyrylthiocholine chloride (Sigma St. Louis, MO, USA), 5,5′-dithiobis [2-nitrobenzoic acid] (DTNB) (Sigma), galantamine hydrobromide from Lycoris sp. (Sigma St. Louis, MO, USA). Instrumentation: (Shimadzu, UV-1700).

Demirci B., Yusufoglu H.S., Tabanca N., Temel H.E., Bernier U.R., Agramonte N.M., Alqasoumi S.I., Al-Rehaily A.J., Başer K.H, & Demirci F. (2016). Rhanterium epapposum Oliv. essential oil: Chemical composition and antimicrobial, insect-repellent and anticholinesterase activities. Saudi Pharmaceutical Journal : SPJ, 25(5), 703-708.

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Malathion Exposure and Butyrylcholinesterase Rescue

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Malathion (MT, Sigma-Aldrich, St. Louis, MO, USA) was diluted in 5% Dimethyl sulfoxide (DMSO) in iCell Neural Complete Maintenance Medium, and then further diluted to concentrations of 10⁻¹, 10⁻³, and 10⁻⁵ M in the same medium. On the 5th day of 3D co-culture, the medium was removed from each well and replaced with 100 μL (50 μL in/outlet) MT-containing medium in replicates of 3 for each MT concentration. After 20 min of MT exposure, one set of replicates was treated with 10 μL (5 μL in/outlet) of 50 μM Butyrylcholinesterase (BuChE, Sigma-Aldrich, St. Louis, MO, USA) in medium. After 24 h, the medium was removed from each well and stored at −20 °C for analysis of ChE activity.

Liu L., Koo Y., Russell T., Gay E., Li Y, & Yun Y. (2020). Three-dimensional brain-on-chip model using human iPSC-derived GABAergic neurons and astrocytes: Butyrylcholinesterase post-treatment for acute malathion exposure. PLoS ONE, 15(3), e0230335.

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