Rat kidney proximal tubular cells (RPTCs) were originally obtained from Dr Hopfer (Case Western Reserve University) and were cultured in DMEM/F12 medium (12400024; Gibco) containing transferrin (5 μg/mL) (T8158; Sigma), insulin (5 μg/mL) (I9278; Sigma), epidermal growth factor (EGF; 1 ng/mL) (E9644; Sigma), dexamethasone (4 μg/mL) (D1756; Sigma), 10% foetal bovine serum (FBS) (PAN‐Seratech) and 1% antibiotic‐antimycotic (15240062; Thermo Fisher Scientific). RPTC HNF1β knockdown and scramble cell lines were constructed in our previous study.17 Lipofectamine 3000 Transfection Regent (L3000008) was purchased from Thermo Fisher Scientific Company.
About 8‐week‐old C57BL/6J male mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (China). Mice were acclimated for 7 days before formal experiments and were housed in groups of four animals per cage. Animal experiments were performed in accordance with the approval of the Ethics Committee for Experimental Animals in Henan University (Approval Number: DWLL20200102).
About 8‐week‐old C57BL/6J male mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (China). Mice were acclimated for 7 days before formal experiments and were housed in groups of four animals per cage. Animal experiments were performed in accordance with the approval of the Ethics Committee for Experimental Animals in Henan University (Approval Number: DWLL20200102).