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Murine il 12 p70

Manufactured by Thermo Fisher Scientific
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Murine IL-12 p70 is a laboratory reagent used for research purposes. It is a cytokine that plays a role in the immune system of mice. This product is intended for use in research applications.

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Lab products found in correlation

Recombinant mouse il 18, by BioLegend (1 mentions) Collagenase 1, by Thermo Fisher Scientific (1 mentions) Murine il 2, by Thermo Fisher Scientific (1 mentions) Isotype control antibody, by BD (1 mentions) Anti parp h 250, by Santa Cruz Biotechnology (1 mentions) Anti tlr9, by Abcam (1 mentions) Glial fibrillary acidic protein (gfap), by Abcam (1 mentions) Anti gapdh, by GeneTex (1 mentions)

2 protocols using murine il 12 p70

1

Cell Surface and Intracellular Staining

Cited 1 time
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The fluorescently conjugated antibodies which were used for cell-surface staining and intracellular staining are listed in the Supplementary Table 1. CD1d-PBS57 (conjugated with PE) was obtained from the tetramer facility of the US National Institutes of Health as previously described27 (link). Collagenase I used for tissue digestion was purchased from Gibco. Murine IL-2 and murine IL-12 p70 were obtained from PeproTech. Recombinant Mouse IL-18 was obtained from BioLegend.
Shen H., Gu C., Liang T., Liu H., Guo F, & Liu X. (2020). Unveiling the heterogeneity of NKT cells in the liver through single cell RNA sequencing. Scientific Reports, 10, 19453.
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2

Immunomodulatory Antibodies and Cytokines

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The antibodies used in this study are listed as follows: anti-IL-12p40 (H306), Santa Cruz Biotechnology, Cat# SC-7926; GFAP, abcam, AB53554; GFAP (GA5), Cell Signaling Technology, Cat# 3670; anti-TLR9, Abcam, ab134368; anti-PARP (H250), Santa Cruz Biotechnology, Cat# SC-7150; anti-GAPDH, GeneTex, Cat# GTX100118. Primary antibodies used for neutralization include p40 (C17.8), or isotype control antibodies, BD Biosciences. The recombinant proteins used in this study to treat SHS-Y5Y cell lines: Murine IL-12 p70; Murine IL-12 p40 and Murine IL-12 p80, PeproTech.
Lai R.H., Chow Y.H., Chung N.H., Chen T.C., Shie F.S, & Juang J.L. (2022). Neurotropic EV71 causes encephalitis by engaging intracellular TLR9 to elicit neurotoxic IL12-p40-iNOS signaling. Cell Death & Disease, 13(4), 328.
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