Flag f3040

Manufactured by Merck Group

Sourced in United States

The Flag (F3040) is a lab equipment product manufactured by Merck Group. It serves as a core function tool for laboratory applications. The detailed technical specifications and intended use are not available in this factual and unbiased description.

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Lab products found in correlation

Alexa fluor 488 conjugated goat anti mouse igg h l, by Cell Signaling Technology (2 mentions) Alexa fluor 594 conjugated goat anti rabbit igg h l, by Cell Signaling Technology (2 mentions) Mg132 m8699, by Merck Group (2 mentions) 3 methyladenine 3 ma catalogue 189490, by Merck Group (1 mentions)

Spelling variants of this product

F3040 (9 citations) Anti-FLAG (F3040) (2 citations) FLAG tag (F3040) (2 citations)

2 protocols using flag f3040

ASFV Protein Antibody Production and Detection

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Rabbit anti-MGF-505-7R and anti-P30 sera were raised against a recombinant ASFV MGF-505-7R and P30 proteins. Polyclonal rabbit anti STAT1 (#14994), phospho-STAT1 (Tyr701) (#9167), JAK1 (#29261), and JAK2 (#3230) were purchased from Cell Signaling Technology. Polyclonal rabbit anti RNF125 (ab211450) and Hes5 (ab194111) were purchased from Abcam. Monoclonal mouse anti HA (H3663), β-actin (A5441), Myc (SAB1305535), and Flag (F3040) were purchased from Sigma-Aldrich. Alexa Fluor-488-conjugated goat anti-mouse IgG (H + L) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) antibodies were purchased from Cell Signaling Technology. 3-MA (189490) and MG132 (M8699) were purchased from Sigma-Aldrich. Porcine IFN-γ ELISA kit was purchased from Solarbio Life Sciences (SEKP-0010).

Li D., Zhang J., Yang W., Li P., Ru Y., Kang W., Li L., Ran Y, & Zheng H. (2021). African swine fever virus protein MGF-505-7R promotes virulence and pathogenesis by inhibiting JAK1- and JAK2-mediated signaling. The Journal of Biological Chemistry, 297(5), 101190.

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Antibody Validation and Small Molecule Inhibitors

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Rabbit anti-p72 was raised against recombinant ASFV p72 protein. Polyclonal rabbit anti-JAK1 (29261), phospho-JAK1 (Tyr1034/1035) (3331S), JAK2 (3230), TYK2 (9312S), phospho-TYK2 (Tyr1054/1055) (9321S), STAT1 (14994), phospho-STAT1 (Tyr701) (9167), STAT2 (D9J7L), and phospho-STAT2 (Y690) (AP0284) were purchased from Cell Signaling Technology (USA). Polyclonal rabbit anti-HERC5 was purchased from Boster Bio. Monoclonal mouse anti-HA (H3663), β-actin (A5441), Myc (SAB1305535), and Flag (F3040) were purchased from Sigma-Aldrich (USA). Monoclonal mouse anti-GFP (sc-9996) was purchased from Santa Cruz Biotechnology (USA). Alexa Fluor-488-conjugated goat anti-mouse IgG (H + L) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) antibodies were purchased from Cell Signaling Technology. 3-Methyladenine (3-MA) (Catalogue #189490) and MG132 (M8699) were purchased from Sigma-Aldrich.

Li D., Peng J., Wu J., Yi J., Wu P., Qi X., Ren J., Peng G., Duan X., Ru Y., Liu H., Tian H, & Zheng H. (2023). African swine fever virus MGF-360-10L is a novel and crucial virulence factor that mediates ubiquitination and degradation of JAK1 by recruiting the E3 ubiquitin ligase HERC5. mBio, 14(4), e00606-23.

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