P paxillin

Antibodies used in this study are: LMO7 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), p-FAK (Cell Signaling, Danvers, MA, USA), Emerin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), β-catenin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), NKCC2 Cell Signaling, Danvers, MA, USA), E-cadherin (Arigo, HsinChu, Taiwan), β-actin (Arigo, HsinChu, Taiwan), ZO-1 (Thermo Fisher Scientific, Waltham, MA, USA), and p-Paxillin (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Specimens were imaged with DM16000B epifluorescence microscopy (Leica, Wetzlar, Germany). Images were analyzed with Software Image-Pro Plus 8.0 (Media Cybernetics, Rockville, MD, USA). Detailed material information is listed in the key resource table (Supplementary Table S3).

Cell lysates were separated by SDS-PAGE in 8–10% gels and transferred into PVDF membranes. Antibodies used were: p-FAK^Y397 (611807), FAK (610088) (BD Transduction Laboratories, Lexington, KY); p-FAK (Tyr³⁹⁷) (sc-11765-R), cortactin (H-191), p-cortactin (Tyr⁴⁶⁶), paxillin (T-16), p-paxillin (Tyr¹¹⁸), E-cadherin (G-10), vimentin (E-5), actin (C-11) (Santa Cruz Biotechnology); α-Tubulin (T9026) (Sigma Aldrich); N-WASP (30D10) (Cell Signaling Technology); p-N-WASP (Ser^484/485) (Chemicon International); p-Arp2 (Thr237) (Biorbyt). Primary and secondary antibodies were incubated with the membranes using standard techniques. Immunodetection was accomplished using enhanced chemiluminescence and recorded with a quantitative digital imaging system (Chemidoc XRS with Image Lab, Bio-Rad, USA).