ELISA kit (E-EL-H1554c, Chian, Wu Han, Elabscience) was used to detect serum HMGB-1 levels in CRC patients. 100 mL/well of standards and samples were loaded into a 96-well plate, and after incubation with biotinylated antibodies, streptavidin-conjugated horseradish peroxidase (HRP) was added to each well and mixed with the HRP substrate solution. The optical density (OD) values were read by a microplate reader at 450 nm.
E el h1554c
Manufactured by Elabscience
Sourced in China
The E-EL-H1554c is a laboratory equipment product from Elabscience. It is a device designed for a specific core function, but without further details or interpretation about its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Il1β elisa kit, by ABclonal (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
E el h6005, by Elabscience (1 mentions)
E el h0149c, by Elabscience (1 mentions)
E el r0012c, by Elabscience (1 mentions)
E el r0505c, by Elabscience (1 mentions)
E el h5812c, by Elabscience (1 mentions)
5 protocols using e el h1554c
1
HMGB-1 Serum Level Quantification
2
HMGB1 and IL1β Quantification in Liver Cancer Cells
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The concentration of High Mobility Group Protein 1 (HMGB1) and IL1β in the supernatant of QGY-7703 and MHCC-97L cells was assayed by ELISA. After transfection for 24 h, the medium was replaced by DMEM (Gibco, Thermo Fisher Scientific) containing 2% FBS (Gibco), and 48 h post-transfection, supernatants were harvested and assayed by HMGB1 (E-EL-H1554c, Elabscience, Hubei, China) and IL1β ELISA kits (RK00001, Abclonal, Hubei, China) following the manufacturer’s instructions.
3
Quantification of Inflammatory Mediators in Rats
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Twenty-four hours after the last administration, 2 mL of venous blood was collected from each experimental rat by subclavian venipuncture. The concentrations of TgAbs, HMGB1, TNF-α, IL-6, IL-1β and monocyte chemoattractant protein-1 (MCP-1) in rats were detected with ELISA kits according to the manufacturer’s protocol (TgAbs: #m1003044, Kangting, Tianjin, China; HMGB1: E-EL-R0505c, Elabscience, China; TNF-α: EK382/3-96, Multisciences, China; IL-6: ER306/3-96, China; IL-1β: E-EL-R0012c, Elabscience, China; MCP-1: E-EL-R0633c, Elabscience, China). The secretion of HMGB1, TNF-α, IL-6, IL-1β and MCP-1 by cell lines was detected with ELISA kits according to the manufacturer’s protocol (HMGB1: E-EL-H1554c, Elabscience, China; TNF-α, EK182HS-48, Multisciences, China; IL-6, EK106/2-48, Multisciences, China; IL-1β: E-EL-H0149c, Elabscience, China; MCP-1: E-EL-H6005, Elabscience, China).
4
Serum Biomarkers in Psoriasis
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The serum concentrations of HMGB1, TLR4, IL‐23, and IL‐17A of psoriasis patients and healthy controls were measured by ELISA according to the manufacturer's instructions for three repeated experiments (cat. no E‐EL‐H1554C, E‐EL‐H5820C, E‐EL‐H0107C, and E‐El‐H5812C; Wuhan Elabscience Biotechnology).
5
HMGB1 Quantification in Serum and Urine
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Both serum and urine HMGB1 levels were detected using the human high-mobility group protein B1 (HMGB1) enzyme-linked immunosorbent assay (ELISA) kit (E-EL-H1554c, Elabscience), and ELISA was performed according to the manufacturer’s instructions. The coefficient of inter-assay and intra-assay variation was <10%. All measurements were repeated thrice horizontally, and the average of these values was used to prevent measurement bias. Additionally, urine HMGB1 levels were corrected for urinary creatinine levels.
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