Sections were incubated with the rat anti-BrdU (1:2000, Serotec, UK) at 4 °C for 24 h. BrdU-positive nuclei were visualized using goat anti-rat-Cy3. After a short fixation step of 10 min, sections were rinsed with PBS and incubated with mouse guinea pig-DCX (1:3000, Millipore, Germany) followed by goat anti-rat-Cy2.
Goat anti rat cy3
Manufactured by Bio-Rad
Sourced in United Kingdom
Goat anti-rat-Cy3 is a secondary antibody conjugated with the fluorescent dye Cy3. It is designed to detect and visualize primary rat antibodies in immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using goat anti rat cy3
1
BrdU and DCX Immunostaining in Rat Tissue
2
Immunolabeling of BrdU and Tubulin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sections were incubated with the rat anti-BrdU (1:2000, Serotec, UK) at 4 °C for 24 h. BrdU-positive nuclei were visualized using goat anti-rat-Cy3. After a short fixation step of 10 min, sections were rinsed with PBS and incubated with rabbit anti-tubulin beta III (1:5000; abcam, UK) followed by goat anti-rat-Cy2.
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