Liver tissue samples were fixed in 0.1 m cacodylate‐buffered Karnovsky fixative containing 2.5% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) overnight at room temperature with a subsequent postfixation in 1% osmium tetroxide (Electron Microscopy Sciences), which was applied for 2 h. Next, the samples were dehydrated in graded ethanol (Sigma‐Aldrich). Afterward, they were embedded in an EMbed‐812 epoxy resin (Electron Microscopy Sciences). Following 2 days of heat polymerization at a temperature of 60 °C, 0.8 µm thin sections were prepared. These were stained with toluidine blue (Agar Scientific; Essex, UK) and basic fuchsine solution (Polysciences Inc.; Warrington, PA, USA). Subsequently, the epon block was adjusted to allow ultrathin sectioning. Eighty‐nm sections were cut with a diamond knife on a Reichert Ultracut‐S ultramicrotome (Leica, Wetzlar, Germany). These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc., Morristown, NJ, USA) and lead citrate solutions (Leica) for 10 min each. A LEO912AB transmission electron microscope (Zeiss, Oberkochen, Germany) operated at 100 kV was used for imaging the ultrathin sections.
Basic fuchsine solution
Manufactured by Polysciences
Sourced in United Kingdom
Basic fuchsine solution is a laboratory reagent used for various analytical and staining applications. It is a concentrated dye solution that can be diluted as needed for specific protocols. The solution contains the dye basic fuchsine, which is a synthetic dye commonly used in histology, microbiology, and other scientific fields. The core function of this product is to provide a standardized and stable dye solution for research and laboratory use.
Automatically generated - may contain errors
Lab products found in correlation
Reichert ultracut s ultramicrotome, by Leica (2 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions)
Osmium tetroxide, by Electron Microscopy Sciences (2 mentions)
Embed812 epoxy resin, by Electron Microscopy Sciences (2 mentions)
Leo 912ab transmission electron microscope, by Zeiss (2 mentions)
Toluidine blue, by Agar Scientific (2 mentions)
Lead citrate solutions, by Leica (2 mentions)
Graded ethanol, by Merck Group (1 mentions)
Glutaraldehyde, by Electron Microscopy Sciences (1 mentions)
Uranyl acetate, by Honeywell (1 mentions)
2 protocols using basic fuchsine solution
1
Ultrastructural Analysis of Liver Tissue
2
Transmission Electron Microscopy of Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissue samples were fixed in 0.1 M cacodylate-buffered Karnovsky fixative containing 2.5% glutaraldehyde and 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) overnight at room temperature with a subsequent post-fixation in 1% osmium tetroxide (Electron Microscopy Sciences), which was applied for 2 h.
Next, the samples were dehydrated in graded ethanols (Sigma Aldrich). Afterward, (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. they were embedded in an EMbed-812 epoxy resin (Electron Microscopy Sciences).
Following two days of heat polymerization at a temperature of 60°C, 0.8 µm thin sections were prepared. These were stained with toluidine blue (AgarScientific; Essex, United Kingdom) and basic fuchsine solution (Polysciences Inc.; Warrington, PA). Subsequently, the epon block was adjusted to allow ultrathin sectioning. Eighty nm sections were cut with a diamond knife on a Reichert Ultracut-S ultramicrotome (Leica, Wetzlar, Germany). These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each. A LEO912AB transmission electron microscope (Zeiss, Oberkochen, Germany) operated at 100 kV was used for imaging the ultrathin sections.
Next, the samples were dehydrated in graded ethanols (Sigma Aldrich). Afterward, (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. they were embedded in an EMbed-812 epoxy resin (Electron Microscopy Sciences).
Following two days of heat polymerization at a temperature of 60°C, 0.8 µm thin sections were prepared. These were stained with toluidine blue (AgarScientific; Essex, United Kingdom) and basic fuchsine solution (Polysciences Inc.; Warrington, PA). Subsequently, the epon block was adjusted to allow ultrathin sectioning. Eighty nm sections were cut with a diamond knife on a Reichert Ultracut-S ultramicrotome (Leica, Wetzlar, Germany). These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each. A LEO912AB transmission electron microscope (Zeiss, Oberkochen, Germany) operated at 100 kV was used for imaging the ultrathin sections.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!