Ab189916

Conventional protocols were used for IB with the details available elsewhere. The following primary antibodies were used for IB: anti-HERPUD1 (1:1000, ab150424, Abcam, Cambridge, UK), anti-GCLC (1:1000, ab190685, Abcam), anti-CBS (1:1000, ab140600, Abcam), anti-CTH (1:1000, ab189916, Abcam), anti-SHMT2 (1:1000, #33443, Cell Signaling Technology (CST), Boston, MA, USA), anti-GSS (1:1000, #ab124811, Abcam) or GSS (H-7) (1:500, sc-166882, Santa Cruz, Heidelberg, Germany), anti-MDM2 (1:1000, ab259265, Abcam), anti-SYVN1 (1:1000, #14773, CST), anti-GAPDH (1:1000, #5174, CST). The membranes were incubated with anti-rabbit IgG, HRP-linked antibody (1:2000, #7071, CST) or anti-mouse IgG, HRP-linked antibody (1:2000, #7076, CST) at room temperature for one hour, and visualized using Clarity Western ECL substrate. The relative expression levels of proteins were determined by densitometry using ImageJ software and were normalized to housekeeping protein.

Primary antibodies: α-actin (A5441, Sigma-Aldrich), α-CSE (ab189916, Abcam), α-ETHE1 (GTX115707, GeneTex), α-GPX4 (ab125066, Abcam), α-MPST (PA5-51548, Thermo Fisher Scientific), α-SQR (ab71978, Abcam) and α-xCT (26864-1-AP, Proteintech). All primary antibodies were used at a dilution of 1:500, except for α-actin (1:1,000). HRP-conjugated antibodies: anti-mouse (115-035-146, Jackson ImmunoResearch) and anti-rabbit (111-035-144, Jackson ImmunoResearch). Secondary antibodies were used at a dilution of 1:10,000.