PBMC were isolated with Ficoll-Isopaque (Lymphoprep™; Axis-Shield, Oslo, Norway) gradient centrifugation. All cells were freshly stained with the following monoclonal antibodies and analysed promptly as previously described (9 (link)): FITC-conjugated anti-CD45RO (UCHL1; BD Biosciences); PE-conjugated anti-CD25 (M-A251; BD Biosciences); PerCP-Cy5.5-conjugated anti-CD4 (SK3; BD Biosciences); and biotinylated anti-FOXP3 (236A/E7; eBioscience, San Diego, CA) and allophycocyanin–streptavidin (BD Biosciences). The second dataset (Table I ) was obtained by LSR Fortessa (BD Biosciences), using the following settings for voltage: FSC-A (319), SSC-A (335), FL1-A (CD45RO, 582), FL2-A (CD25, 478), FL3-A (CD4, 742), and FL4-A (FOXP3, 676). FlowJo (Tree Star) was used for manual gating.
Fl4 a
Manufactured by BD
The FL4-A is a compact and versatile flow cytometry instrument designed for accurate cell analysis. It features four-channel detection capabilities, enabling the simultaneous measurement of multiple parameters. The FL4-A is a reliable tool for various applications in life science research and clinical diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin allophycocyanin, by BD (2 mentions)
Ficoll isopaque lymphoprep, by Axis-Shield (2 mentions)
Fitc conjugated anti cd45ro uchl1, by BD (2 mentions)
Pe conjugated anti cd25 m a251, by BD (2 mentions)
Percp cy5.5 conjugated anti cd4 sk3, by BD (2 mentions)
Biotinylated anti foxp3 236a e7, by Thermo Fisher Scientific (2 mentions)
Fsc a, by BD (2 mentions)
Ssc a, by BD (2 mentions)
Fl1 a, by BD (2 mentions)
Fl2 a, by BD (2 mentions)
Fl3 a, by BD (2 mentions)
Lsrfortessa, by BD (2 mentions)
2 protocols using fl4 a
1
Phenotypic Analysis of T-Cell Subsets
2
Isolation and Phenotyping of Human PBMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PBMCs were isolated with Ficoll–Isopaque (Lymphoprep; Axis-Shield, Oslo, Norway) gradient centrifugation. All cells were freshly stained with the following mAbs and analyzed promptly as previously described (9 (link)): FITC-conjugated anti-CD45RO (UCHL1; BD Biosciences); PE-conjugated anti-CD25 (M-A251; BD Biosciences); PerCP-Cy5.5–conjugated anti-CD4 (SK3; BD Biosciences); and biotinylated anti-FOXP3 (236A/E7; eBioscience, San Diego, CA) and allophycocyanin-streptavidin (BD Biosciences). The second data set (Table I ) was obtained by LSR Fortessa (BD Biosciences), using the following settings for voltage: FSC-A (319), SSC-A (335), FL1-A (CD45RO, 582), FL2-A (CD25, 478), FL3-A (CD4, 742), and FL4-A (FOXP3, 676). FlowJo (Tree Star) was used for manual gating.
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