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Tlc silica gel 60 f254 glass plates

Manufactured by Merck Group
Sourced in Germany

TLC silica gel 60 F254 glass plates are a type of thin-layer chromatography (TLC) product manufactured by Merck Group. They consist of a silica gel 60 adsorbent layer coated on a glass support, with the addition of a fluorescent indicator (F254) that enables visualization of separated compounds under UV light. These plates are designed for the separation, identification, and purification of a wide range of organic and inorganic compounds.

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9 protocols using tlc silica gel 60 f254 glass plates

1

Synthesis and Purification of Novel Substrates

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All chemicals were purchased from
commercial sources or provided by Pfizer and used without further
purification. Substrates 2bd were
synthesized according to the reported procedure. Unless stated otherwise,
all column purification were performed on a Biotage Selekt Flash Chromatography,
eluted with ethyl acetate: hexane, 0–15% gradient, detected
by UV absorption at 254 nm. Thin layer chromatography (TLC) was carried
out using Merck Millipore TLC silica gel 60 F254 glass plates. 1H, 13C, and 19F NMR spectra were measured
on a Bruker DPX-400 instrument (operating at 400 MHz for 1H, 100 MHz for 13C, and 375 MHz for 19F) or
a Bruker DPX-500 instrument (operating at 500 MHz for 1H and 125 MHz for 13C).
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2

Synthesis and Characterization of Chiral Nematic LC Dopants

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A nematic LC mixture, JC‐1041XX (JNC Co., Δn=0.142, Δϵ=5.7 at 25 °C) was used as the host LC. All chemical products to synthesize the chiral dopants and solvents were purchased from commercial suppliers and used without further purification. Column chromatography was carried out on SiO2 60 n (particle size 0.063–0.210 mm; Kanto Chemical Co.). Thin‐layer chromatography was performed on TLC silica gel 60 F254 glass plates (Merck). 1H and 13C NMR spectra were recorded at 25 °C in CDCl3 as a solvent by using a JEOL JNM‐LA400 (400 MHz) instrument and trimethylsilane as the initial standard. Mass spectra (MS) and elemental analyses were obtained from the Service Center at Kyushu University. UV/Vis and CD spectra were recorded in MeCN by using an UV/VIS/NIR spectrophotometer (SHIMADZU, UV‐3150) and spectropolarimeter (JASCO Co., J‐720W), respectively. Photoisomerization was conducted by using visible light from a Xenon light source (300 W, ASAHI SPECTRA Co.) through band‐pass filters (λ=400, 460, and 600 nm, ASAHI SPECTRA Co.). Microscopic analysis was carried out by using a Nikon ECLIPSE E600 POL optical microscope.
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3

Synthesis and Characterization of Porphyrin Derivatives

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All the chemicals and reagents were purchased from commercial suppliers (Sigma-Aldrich, Alfa Aesar) and used without any further purification, unless otherwise stated. Mn- and Co-protoporphyrin IX, protoporphyrin IX dimethyl ester, and mesoporphyrin IX dimethyl ester were purchased from Frontier Scientific. EDA was purchased from Sigma-Aldrich as 87% solution in dichloromethane. All dry reactions were carried out under argon or nitrogen in oven-dried glassware with magnetic stirring using standard gas-tight syringes, cannulae and septa. 1H and 13C NMR spectra were measured on Bruker DPX-400 (operating at 400 MHz for 1H and 100 MHz for 13C) or Bruker DPX-500 (operating at 500 MHz for 1H and 125 MHz for 13C). Tetramethylsilane (TMS) (0 ppm) or D2O (4.6 ppm) or d6-DMSO (2.50 ppm) served as the internal standard for 1H NMR and CDCl3 (77.0 ppm) or d6-DMSO (39.51 ppm) was used as the internal standard for 13C NMR. Silica gel chromatography purifications were carried out using AMD Silica Gel 60 230–400 mesh. Thin Layer Chromatography (TLC) and preparative TLC were carried out using Merck Millipore TLC silica gel 60 F254 glass plates.
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4

Carbohydrate-based Analytical Procedures

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Maltooligosaccharide (G1-4), soluble potato starch, corn starch, bovine serum albumin and ampicillin sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tryptone and yeast extract were obtained from Difco (Bacton Dickinson and company, Sparks, MD, USA). β-CD was purchased from AppliChem (Darmstadt, Germany). Standard maltitol and D-sorbitol were obtained from TCI (Toshima, Kita-ku, Tokyo, Japan). Aquacide II, methanol, isopropanol, ethyl acetate, acetonitrile, HPLC-grade water, potassium dihydrogen phosphate, dipotassium phosphate and TLC silica gel 60 F254 glass plates (20 cm in height) were purchased from Merck (Darmstadt, Germany). IPTG, glycine and Tris-HCl were obtained from Vivantis (Sendirian Berhad, Shah Alam, Malaysia). Sulfuric acid was obtained from Carlo Erba Reagents (Val de Reuil, France). The commercial glucose oxidase kit was obtained from Human mit-diagnostics GmbH (Idstein, Germany). Other chemicals used were of an analytical grade.
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5

Synthesis and Characterization of Fluorinated Substrates

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All chemicals were purchased from commercial sources or provided by Pfizer and used without further purification. Substrates 2b-2d were synthesized according to the reported procedure. Unless stated otherwise, all column purification were performed on a Biotage Selekt Flash Chromatography, eluted with ethyl acetate: hexane, 0-15% gradient, detected by UV absorption at 254nm. Thin Layer Chromatography (TLC) was carried out using Merck Millipore TLC silica gel 60 F254 glass plates. 1H, 13C, and 19F NMR spectra were measured on a Bruker DPX-400 instrument (operating at 400 MHz for 1H, 100 MHz for 13C, and 375 MHz for 19F) or a Bruker DPX-500 instrument (operating at 500 MHz for 1H and 125 MHz for 13C).
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6

Highly Enantioselective Cyclopropanation of Olefins

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All chemicals and reagents were purchased from commercial suppliers (Sigma-Aldrich, Alfa Aesar, TCI, Oakwood Chemicals, Combi Blocks) and used without any further purification, unless otherwise stated. EDA was purchased from Sigma-Aldrich as 87% m/v solution in dichloromethane. Dimethyl(4-vinylphenyl)silane 3a and 4-(dimethylsilyl)aniline 4a were synthesized according to a previously reported procedure.6 Authentic racemic standards for the cyclopropanation products 7b-15b and 20b were synthesized and characterized as described previously.2 (link)a,2 (link)b All dry organic reactions were carried out under argon in oven-dried glassware with magnetic stirring using standard gas-tight syringes, cannulae and septa. 1H and 13C NMR spectra were measured on Bruker DPX-400 (operating at 400 MHz for 1H and 100 MHz for 13C) or Bruker DPX-500 (operating at 500 MHz for 1H and 125 MHz for 13C). Tetramethylsilane (TMS) served as the internal standard (0 ppm) for 1H NMR and the residual proton signal of the solvents was used as the internal standard for 13C NMR. HRMS analyses were performed using a Q Exactive Plus Mass Spectrometer at the Proteomics Facility of the University of Rochester. Silica gel chromatography purifications were carried out using AMD Silica Gel 60 230–400 mesh. Thin Layer Chromatography (TLC) were carried out using Merck Millipore TLC silica gel 60 F254 glass plates.
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7

Characterization of Organic Compounds

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All the chemicals and reagents
were purchased from commercial suppliers (Sigma-Aldrich, Alfa Aesar,
ACS Scientific, Acros, Ambeed, Combi-blocks) and used without any
further purification. All dry reactions were carried out under argon
in flame-dried glassware with magnetic stirring using standard gastight
syringes, cannula, and septa. 1H and 13C NMR
spectra were measured on Bruker DPX-500 (operating at 500 MHz for 1H and 125 MHz for 13C) or Bruker DPX-400 (operating
at 400 MHz for 1H and 100 MHz for 13C), and 19F was measured on Bruker DPX-400 (operating at 375 MHz).
Tetramethylsilane (TMS) (0 ppm) and/or CDCl3 (7.26 ppm)
served as the internal standard for 1H NMR, CDCl3 was used as the internal standard (77.0 ppm) for 13C
NMR, and trifluorotoluene served as the internal standard (−63
ppm) for 19F NMR. HRMS analyses were performed using a
Q Exactive Plus mass spectrometer at the Proteomics Facility of the
University of Rochester. Silica gel chromatography purifications were
carried out using AMD silica gel 60 230–400 mesh. Thin layer
chromatography (TLC) was carried out using Merck Millipore TLC silica
gel 60 F254 glass plates.
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8

Optimized Organic Synthesis Procedures

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All the chemicals and reagents were purchased from commercial suppliers (Sigma-Aldrich, Alfa Aesar, ACS Scientific, Acros, Ambeed, Combi-blocks) and used without any further purification. All dry reactions were carried out under argon in flame-dried glassware with magnetic stirring using standard gas-tight syringes, cannula, and septa. 1H and 13C NMR spectra were measured on Bruker DPX-500 (operating at 500 MHz for 1H and 125 MHz for 13C) or Bruker DPX-400 (operating at 400 MHz for 1H and 100 MHz for 13C), 19F was measured on Bruker DPX-400 (operating at 375 MHz). Tetramethylsilane (TMS) (0 ppm) and/or CDCl3 (7.26 ppm) served as the internal standard for 1HNMR, CDCl3 was used as the internal standard (77.0 ppm) for 13CNMR, and trifluorotoluene served as the internal standard (−63 ppm) for 19F NMR. HRMS analyses were performed using a Q Exactive Plus Mass Spectrometer at the Proteomics Facility of the University of Rochester. Silica gel chromatography purifications were carried out using AMD Silica Gel 60 230-400 mesh. Thin Layer Chromatography (TLC) was carried out using Merck Millipore TLC silica gel 60 F254 glass plates.
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9

Standard Organic Chemistry Procedures

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All the chemicals and reagents were purchased from commercial suppliers (Sigma-Aldrich, Alfa Aesar, ACS Scientific, Acros) and used without any further purification. All dry reactions were carried out under argon in flame-dried glassware with magnetic stirring using standard gas-tight syringes, cannula, and septa. 1H and 13C NMR spectra were measured on Bruker DPX-500 (operating at 500 MHz for 1H and 125 MHz for 13C) or Bruker DPX-400 (operating at 400 MHz for 1H and 100 MHz for 13C). Tetramethylsilane (TMS) served as the internal standard (0 ppm) for 1HNMR and CDCl3 was used as the internal standard (77.0 ppm) for 13CNMR. Silica gel chromatography purifications were carried out using AMD Silica Gel 60 230–400 mesh. Thin Layer Chromatography (TLC) was carried out using Merck Millipore TLC silica gel 60 F254 glass plates.
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