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Methotrexate mtx

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Methotrexate (MTX) is a chemical compound used in various laboratory applications. It is a folate antagonist that inhibits the enzyme dihydrofolate reductase, which is involved in the synthesis of DNA and RNA. MTX is commonly used in research applications, including cell culture studies and biochemical assays.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (2 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (2 mentions) Pyridoxamine pm dihydrochloride, by Merck Group (2 mentions) Riboflavin b2, by Thermo Fisher Scientific (2 mentions) Ultrapure water hplc grade, by Thermo Fisher Scientific (2 mentions) Lc ms grade acetonitrile, by Thermo Fisher Scientific (2 mentions) Flavin mononucleotide, by Merck Group (2 mentions) 4 pyridoxic acid 4 pa, by Merck Group (1 mentions) Pyridoxine b6, by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Folic acid, by Merck Group (1 mentions) Methylmalonic acid mma, by Merck Group (1 mentions) Tris 2 carboxyethyl phosphine tcep, by Merck Group (1 mentions)

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Methotrexate (2 citations)

2 protocols using methotrexate mtx

1

Quantification of Vitamins and Folates

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Analytical grades of ascorbic acid, flavin mononucleotide (FMN), folic acid (FA), pyridoxine (B6), pyridoxal (PL) hydrochloride, pyridoxamine (PM) dihydrochloride, and 4-pyridoxic acid (4PA) were bought from Sigma (St Louis, MO, USA). Riboflavin (B2) was bought from Alfa Aesar and 5-methyltetrahydrofolate (5MTHF), dihydrofolate (DHF) and tetrahydrofolate (THF) were acquired from Cayman Chemicals (Ann Arbor, MI, USA). Methotrexate (MTX), purchased from Enzo Life Sciences (Farmingdale, NY, USA), was used as an internal standard (IS) for the assay. The purity of each standard was above 97%, except DHF and THF which were 90% and 95% respectively. Ultrapure HPLC-grade water, LCMS-grade methanol, LCMS-grade acetonitrile and formic acid were purchased from Fisher Scientific (Pittsburg, PA, USA) and were used for sample processing and mobile phase systems.
Asante I., Pei H., Zhou E., Liu S., Chui D., Yoo E, & Louie S.G. (2018). Simultaneous quantitation of folates, flavins and B6 metabolites in human plasma by LC–MS/MS assay: Applications in colorectal cancer. Journal of pharmaceutical and biomedical analysis, 158, 66-73.
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2

Comprehensive Metabolite Standard Sourcing

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Analytical grade flavin mononucleotide (FMN), folic acid (FA), pyridoxine (B6), pyridoxal (PL) hydrochloride,
pyridoxamine (PM) dihydrochloride, 4-pyridoxic acid (4PA), zinc sulphate, and tris(2-carboxyethyl)phosphine (TCEP) were purchased
from Sigma (St Louis, MO, USA); riboflavin (B2) was purchased from Alfa Aesar, and 5-methyltetrahydrofolate (5MTHF),
dihydrofolate (DHF) and tetrahydrofolate (THF) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Methotrexate (MTX),
purchased from Enzo Life Sciences (Farmingdale, NY, USA), was used as an internal standard for the assay. The purity of each
standard was above 97%, except DHF and THF which were 90% and 95% respectively. Ultrapure HPLC-grade water, LCMS-grade methanol,
LCMS-grade acetonitrile and formic acid were purchased from Fisher Scientific (Pittsburg, PA, USA) and were used for sample
processing and mobile phase systems. In addition, homocysteine (HCY), methionine (METH), S-adenosyl methionine (SAM), S-adenosyl
homocysteine (SAH), deuterated SAH, cystathionine (CYSTH), methylmalonic acid (MMA), and tris(2-carbixyethyl)phosphine (TCEP) were
bought from Sigma (St Louis, MO, USA). The deuterated MMA was bought from Medical Isotopes (Pelham, NH, USA).
Asante I., Pei H., Zhou E., Liu S., Chui D., Yoo E., Conti D.V, & Louie S.G. (2019). Exploratory Metabolomic Study to Identify Blood-based Biomarkers as a Potential Screen for Colorectal Cancer. Molecular omics, 15(1), 21-29.
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