Cardiopet probnp test

Manufactured by IDEXX

Sourced in Germany, United Kingdom

The Cardiopet proBNP test is a laboratory diagnostic tool designed to measure the levels of N-terminal pro-B-type natriuretic peptide (NT-proBNP) in a patient's blood sample. NT-proBNP is a biomarker that can provide information about the heart's function.

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Lab products found in correlation

Advia centaur xp, by Siemens (2 mentions) Advia centaur tni ultra assay, by Siemens (1 mentions) Au640, by Olympus (1 mentions)

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Cardiopet proBNP (3 citations) Cardiopet (2 citations)

6 protocols using cardiopet probnp test

Canine Natriuretic Peptides Measurement

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Blood samples were collected from the jugular vein at the initial examination in all
dogs. The blood samples were collected in tubes containing aprotinin (for measurement of
atrial natriuretic peptide [ANP]) and ethylene diamine tetraacetic acid (for measurement
of N-terminal pro-B-type natriuretic peptide [NT-proBNP]), then centrifuged at 1,500
g for 10 min at 4°C. The plasma C-terminal ANP concentration was
determined using a chemiluminescence enzyme immunoassay for human α-ANP in a commercial
laboratory (Fujifilm Monolis, Co., Ltd., Tokyo, Japan). The precision of the ANP assay has
been reported previously [18 (link)]. Plasma NT-proBNP
concentrations were determined using an enzyme immunoassay for canine NT-proBNP in a
commercial laboratory (Cardiopet proBNP test; IDEXX Laboratories, Ludwigsburg,
Germany).

HORI Y., NAKAMURA K., KANNO N., HITOMI M., YAMASHITA Y., HOSAKA S., ISAYAMA N, & MIMURA T. (2018). Effects of the angiotensin-converting enzyme inhibitor alacepril in dogs with mitral valve disease. The Journal of Veterinary Medical Science, 80(8), 1212-1218.

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Blood Biomarker Measurement Protocol

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Blood was collected into EDTA tubes by jugular venepuncture at the initial and last assessments. Samples were immediately centrifuged and separated EDTA-plasma stored at -20 °C until after study completion and sent as a single batch on dry ice to an external laboratory (IDEXX Laboratories, Wetherby, West Yorkshire, UK) for measurement of hs-cTnI (Beckman Coulter Access hs-cTnI assay; IDEXX Laboratories) and second-generation NT-proBNP (Cardiopet proBNP test, IDEXX Laboratories).

Partington C., Hodgkiss-Geere H., Woods G.R., Dukes-McEwan J., Flanagan J., Biourge V, & German A.J. (2022). The effect of obesity and subsequent weight reduction on cardiac structure and function in dogs. BMC Veterinary Research, 18, 351.

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Biomarker Measurement in EDTA-Plasma

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Blood was collected into EDTA tubes by jugular venepuncture at the initial and last assessments. Samples were immediately centrifuged and separated EDTA-plasma stored at -20 °C until after study completion and sent as a single batch on dry ice to an external laboratory (IDEXX Laboratories, Wetherby, West Yorkshire, UK) for measurement of high sensitivity cardiac troponin I (Beckman Coulter Access hs-cTnI assay; IDEXX Laboratories) and second-generation N-terminal Pro B-type natriuretic peptide (Cardiopet proBNP test, IDEXX Laboratories).

Partington C., Hodgkiss-Geere H., Woods G.R., Dukes-McEwan J., Flanagan J., Biourge V, & German A.J. (2024). The effect of obesity and subsequent weight reduction on cardiac morphology and function in cats. BMC Veterinary Research, 20, 154.

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Biomarkers

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At enrollment (baseline) and at each subsequent Holter visit, 5 mL of venous blood were collected. The samples were centrifuged, plasma (for NT‐proBNP) and serum (for cTnI and CRP) were separated and collected. At 1 center, samples were stored at −20°C for up to 3 weeks before analysis. At the other centers samples were stored at −80°C until the end of the study. The NT‐proBNP was analyzed using the Cardiopet proBNP test (IDEXX Laboratories, 2nd generation ELISA), the cTnI by ultrasensitive chemiluminescence immunoassay (Advia Centaur TnI‐Ultra assay; Siemens Healthcare Diagnostics) and CRP by immunoturbidimetry.
To determine which clinical, echocardiographic or biomarker variables were important to achieving a meanHR_Holter <125 bpm, echocardiography was performed, and blood was collected for biomarkers measurement at each Holter visit. Only the echocardiographic and biomarker data from the date of Holter_final were analyzed.

Pedro B., Mavropoulou A., Oyama M.A., Linney C., Neves J., Dukes‐McEwan J., Fontes‐Sousa A.P, & Gelzer A.R. (2023). Optimal rate control in dogs with atrial fibrillation—ORCA study—Multicenter prospective observational study: Prognostic impact and predictors of rate control. Journal of Veterinary Internal Medicine, 37(3), 887-899.

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Comprehensive Serum Biochemistry Panel

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One serum aliquot was used for measurement of biochemical parameters using commercial reagents (Beckman Coulter) per manufacturer's instructions in an automatic analyser (Olympus AU 640, Hamburg, Germany). The following parameters were measured: serum urea, creatinine, bilirubin, glucose, proteins, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (AP), creatine kinase (CPK), lactate dehydrogenase (LDH), C-reactive protein (CRP), alpha-amylase, lipase, cholesterol, triglycerides, calcium, phosphates.
Cardiac troponin I (cTnI) was measured using a commercially available ADVIA Centaur TnI-Ultra assay, which is a high-sensitivity immunoassay validated for use in both humans and dogs [10] . Analysis was performed in Dubrava Clinical Hospital, Department of clinical diagnostics (Zagreb, Croatia) using Siemens Advia Centaur XP according to manufacturer's instructions. Samples which had serum concentration of cTnI below the lower level of detection of the assay were allocated a value of 0.01 μg/L. Analysis of N-terminal pro b-type natriuretic peptide (NT-proBNP) concentration in all samples was performed in Vet Med Labor GmbH, reference IDEXX Laboratory (Germany) using IDEXX Cardiopet® proBNP test.

Kuleš J., Bilić P., Horvatić A., Kovačević A., Guillemin N., Ljubić B.B., Galan A., Jović I., Torti M., Rubić I., Eckersall P.D, & Mrljak V. (2020). Serum proteome profiling in canine chronic valve disease using a TMT-based quantitative proteomics approach. Journal of proteomics, 223.

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Cardiac Biomarkers in Dilated Cardiomyopathy

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Cardiac troponin I (cTnI) was measured in serum samples of control (N=8) and iDCM (N=8) group using commercially available ADVIA Centaur TnI-Ultra assay, which is a high-sensitivity immunoassay validated for use in both humans and dogs [15] . Analysis was performed in Dubrava Clinical Hospital, Department of clinical diagnostics (Zagreb, Croatia) using Siemens Advia Centaur XP according to manufacturer´s instructions. Samples which had serum concentration of cTnI below the lower level of detection of the assay were allocated a value of 0.01 μg/L. Analysis of NT-proBNP concentration in all 16 samples was performed in Vet Med Labor GmbH, reference IDEXX Laboratory (Germany) using IDEXX Cardiopet® proBNP test.

Bilić P., Guillemin N., Kovačević A., Beer Ljubić B., Jović I., Galan A., Eckersall P.D., Burchmore R, & Mrljak V. (2018). Serum proteome profiling in canine idiopathic dilated cardiomyopathy using TMT-based quantitative proteomics approach. Journal of proteomics, 179.

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