For all experiments including the use of drug treatments, drug solvents (vehicle; EtOH or DMSO) were used as controls. Nifedipine (Sigma) was dissolved in EtOH and used at 50 μM for acute experiments, while 5 μM was used for chronic experiments. ω-agatoxin IVA (Alomone Labs, Jerusalem, Israel) was dissolved in distilled water and used at a final concentration of 100 nM. Tetrodotoxin (TTX; Alomone Labs) was dissolved in distilled water and used at final concentrations between 2.5 and 5 μM. D-tubocurarine chloride hydrate (Sigma) was dissolved in distilled water and used at 50 μM. Dynasore (Sigma) was dissolved in DMSO and used at a final concentration of 90 μM.
Dtubocurarine chloride hydrate
Manufactured by Merck Group
Sourced in Israel
Dtubocurarine chloride hydrate is a chemical compound used as a laboratory reagent. It functions as a neuromuscular blocking agent, which temporarily prevents muscle contraction by inhibiting the action of acetylcholine at the neuromuscular junction.
Automatically generated - may contain errors
3 protocols using dtubocurarine chloride hydrate
1
Pharmacological Modulation of Neuronal Activity
2
Calcium Imaging of Zebrafish Trigeminal Nerves
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Briefly, FAM19A5l:gal4vp16 BAC DNA was micro-injected into one-cell Tg(uas:GCaMP6s);nacre embryos. Zebrafish larvae expressing GCaMP6s in TGs were embedded in 2 % low-melting agarose in a dish containing E3 medium with 100 μM Dtubocurarine chloride hydrate (Sigma; T2379). Time-lapse recording was carried out under 50 msec exposure at 20 fps for 280 s using a spinning disc confocal microscope (CSU-X1, Nikon). Larvae were treated with 100 μM allyl isothiocyanate (mustard oil, AITC, 377430, Sigma-Aldrich) at 100 s. The images obtained were analyzed using NIS-Elements software.
3
Calcium Imaging of Zebrafish Trigeminal Nerves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, FAM19A5l:gal4vp16 BAC DNA was micro-injected into one-cell Tg(uas:GCaMP6s);nacre embryos. Zebrafish larvae expressing GCaMP6s in TGs were embedded in 2 % low-melting agarose in a dish containing E3 medium with 100 μM Dtubocurarine chloride hydrate (Sigma; T2379). Time-lapse recording was carried out under 50 msec exposure at 20 fps for 280 s using a spinning disc confocal microscope (CSU-X1, Nikon). Larvae were treated with 100 μM allyl isothiocyanate (mustard oil, AITC, 377430, Sigma-Aldrich) at 100 s. The images obtained were analyzed using NIS-Elements software.
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