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Ovalbumin (ova)

Manufactured by Mimotopes

Ovalbumin is a protein found in egg white. It is commonly used as a standard or control in various laboratory techniques and assays.

Automatically generated - may contain errors

2 protocols using ovalbumin (ova)

1

Anti-P2X7 Antibody Production Protocol

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Example 1

Anti-nonfunctional P2X7 antibody was raised against the peptide KYYKENNVEKRTLIKVF (SEQ ID NO: 2) or Lys-Tyr-Tyr-Lys-Glu-Asn-Asn-Val-Glu-Lys-Arg-Thr-Leu-Ile-Lys-Val-Phe, representing amino acids 297-313 of the human P2X7 protein. The peptide was synthesized by solid phase chemistry (Mimotopes Pty Ltd, Melbourne, Australia) to high purity (>95%) as judged by mass spectroscopy. A C-terminal Cys (C) residue was attached to the sequence and to this was attached the cross-linker MCS (6-Maleimido-Caproic Acid N-Hydroxysuccinimide Ester) for conjugating the peptide to carrier proteins including separately Diphtheria toxoid, bovine serum albumin and ovalbumin by Mimotopes.

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2

Antibody Generation for P2X7 Receptor

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 1

Anti-nonfunctional P2X7 antibody was raised against the peptide KYYKENNVEKRTLIKVF (SEQ ID NO: 2) or Lys-Tyr-Tyr-Lys-Glu-Asn-Asn-Val-Glu-Lys-Arg-Thr-Leu-Ile-Lys-Val-Phe, representing amino acids 297-313 of the human P2X7 protein. The peptide was synthesized by solid phase chemistry (Mimotopes Pty Ltd, Melbourne, Australia) to high purity (>95%) as judged by mass spectroscopy. A C-terminal Cys (C) residue was attached to the sequence and to this was attached the cross-linker MCS (6-Maleimido-Caproic Acid N-Hydroxysuccinimide Ester) for conjugating the peptide to carrier proteins including separately Diphtheria toxoid, bovine serum albumin and ovalbumin by Mimotopes.

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