Anti p h3

Manufactured by Abcam

Anti-p-H3 is a lab equipment product designed to detect and measure phosphorylated histone H3 (p-H3), a post-translational modification of the histone H3 protein. This product can be used in various applications related to cell cycle analysis and chromatin structure studies.

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Anti-H3 (148 citations) Anti-p-H3 (Ser10) (2 citations)

4 protocols using anti p h3

Molecular Mechanisms in Inflammatory Response

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Angiotensin II and JSH-23 were purchased from Sigma-Aldrich (St. Louis, MO). The primary antibodies were obtained from the following sources: anti-LC3, anti-p-p65 NF-κB (Ser536), and anti-p65 NF-κB (Cell Signaling Technology, Beverly, MA), anti-ATG5, anti-IL-1β, anti-F4/80, anti-CD3, and anti-p-H3 (Abcam, Cambridge, MA); Anti-p62 GeneTex (Irvine, CA); anti-FLAG and anti-HA (Invitrogen Life Technologies, Paisley, UK); Alexa Fluor 488 goat anti-rabbit IgG antibody, Alexa Fluor 488 goat anti-mouse IgG antibody, Alexa Fluor 546 goat anti-mouse IgG antibody, and Alexa Fluor546 goat anti-rabbit IgG antibody (Invitrogen Life Technologies, Paisley, UK).

Peng X., Wang Y., Li H., Fan J., Shen J., Yu X., Zhou Y, & Mao H. (2019). ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury. Cell Death & Disease, 10(4), 253.

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Immunostaining of Drosophila Tissues

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Larval and adult tissues were fixed for 20 min in 4% formaldehyde in PBS and immunostained as previously described (Bello et al, 2003). The primary antibodies used were anti‐Mira (mouse, 1:50, gift of F. Matzusaki), anti‐GFP (chick, 1:2,000, Abcam), anti‐pH3 (rat, 1:500, Abcam), anti‐Dpn (rabbit, 1:100, gift of Y.N. Jan), anti‐Dpn (guinea pig, 1:1,000, gift of James Skeath), anti‐Ase (rabbit, 1:50, gift of F. Matsuzaki), anti‐Elav (mouse or rat, 1:100, Developmental Studies Hybridoma Bank), anti‐Myc (rabbit, 1:100, Santa Cruz) and anti‐Fib (mouse, 1:200, Abcam). Secondary goat antibodies conjugated to Alexa488, Alexa568, Alexa650 and Alexa505 (Molecular Probes) were used 1:200. DAPI (Molecular Probes) was used at 1:10,000. Fluorescent images were collected on a Leica SP5 confocal microscope, and all images shown are single sections unless otherwise stated.

Froldi F., Pachnis P., Szuperák M., Costas O., Fernando T., Gould A.P, & Cheng L.Y. (2019). Histidine is selectively required for the growth of Myc‐dependent dedifferentiation tumours in the Drosophila CNS. The EMBO Journal, 38(7), e99895.

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Immunohistochemical Analysis of Tissue Markers

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Immunohistochemistry was performed using 3- to 5-μm-thick tissue sections mounted onto slides that were dewaxed, rehydrated, permeabilized, and blocked. The slides were incubated with primary antibodies, i.e. anti-HIF-1α (1:500, Chemicon), anti-p53 (1:500, Thermo Fisher Scientific), anti-Ki-67 (1:1000, Abcam), anti-p-H3 (1:1000, Abcam), anti-SMA (1:500, Abcam), anti-COL1A1 (1:40, Sangon Biotech. Co.), anti-COL4A1 (1:40, Sangon Biotech. Co.), and anti-fibronectin (1:600, Abcam), at 4°C overnight. The sections were then incubated with appropriate biotinylated secondary antibodies and visualized. Nonimmune goat IgG or rabbit IgG was used as the control. Immunocytochemistry was performed using an established protocol (Sun et al., 2009 (link); Du et al., 2014 (link)).

Liu L., Zhang P., Bai M., He L., Zhang L., Liu T., Yang Z., Duan M., Liu M., Liu B., Du R., Qian Q, & Sun S. (2018). p53 upregulated by HIF-1α promotes hypoxia-induced G2/M arrest and renal fibrosis in vitro and in vivo. Journal of Molecular Cell Biology, 11(5), 371-382.

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Comprehensive Immunostaining of Drosophila Tissues

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Anti-PH3 (1:500; Abcam), anti-CycA [1:50; Developmental Studies Hybridoma Bank (DSHB)], anti-Dcp-1 (1:100; Cell Signaling Technology), anti-Cut (1:10; DSHB), anti-Wg (1:1000; DSHB), anti-Elav (1:50; DSHB) and anti-NICD (1:10; DSHB) were used as the source for primary antibodies. Secondary antibodies included Alexa Fluor 488, 555 and 633 (Invitrogen). For nuclear staining, 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) was used. Standard procedures were followed for immunostaining. The crosses were properly synchronized for the stages and third-instar larvae were collected for dissections. Briefly, fly tissues were dissected and fixed in PBS containing 4% formaldehyde for 30 min. Following double rinsing in PBS containing 0.1% Triton X-100, the samples were incubated overnight at 4°C with the primary antibody. The samples were then blocked with 1% BSA for 2 h followed by a 2-h incubation with secondary antibody at room temperature. Finally, the samples were mounted with Vectashield medium (Vector Laboratories). Images were acquired with a Zeiss LSM 510 confocal microscope and processed using Adobe Photoshop CS6.

Harsh S., Fu Y., Kenney E., Han Z, & Eleftherianos I. (2020). Zika virus non-structural protein NS4A restricts eye growth in Drosophila through regulation of JAK/STAT signaling. Disease Models & Mechanisms, 13(4), dmm040816.

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