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Elisa brdu incorporation

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The ELISA BrdU incorporation is a lab equipment product that measures cell proliferation by detecting the incorporation of the thymidine analog bromodeoxyuridine (BrdU) into the DNA of dividing cells. This assay provides a quantitative assessment of cell proliferation through colorimetric detection.

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Lab products found in correlation

Incucyte s3 live cell analysis system, by Sartorius (2 mentions) Synergy 2, by Agilent Technologies (2 mentions) Prism 7, by GraphPad (2 mentions)

Close spelling variants of this product

ELISA BrdU incorporation assay BrdU incorporation BrdU ELISA ELISAs

2 protocols using elisa brdu incorporation

1

Cell Viability and Proliferation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Treatment responses were measured using crystal violet staining or ELISA BrdU incorporation (Sigma 11669915001) as described by manufacturer. Cell Titer Glo was used to measure ATP levels and CyQuant was used to measure DNA content in treated cultures. Luminescence/fluorescence was read on a BioTek Synergy 2 plate reader. Live cell analysis was performed using 96-well collagen-coated tissue culture treated plates in IncuCyte S3 Live-Cell Analysis System (Essen Biosciences). Essen Bioscience software was used to quantify number of H2B-GFP positive cells per well and then normalized to starting cell number for each condition. Data was exported to Prism 7 (GraphPad) for statistical analysis and graph generation. Each drug treatment was carried out with multiples greater than six and verified in two independent experiments.
Knudsen E.S., Kumarasamy V., Ruiz A., Sivinski J., Chung S., Grant A., Vail P., Chauhan S.S., Jie T., Riall T.S, & Witkiewicz A.K. (2019). Cell cycle plasticity driven by MTOR signaling: Integral resistance to CDK4/6 inhibition in patient-derived models of pancreatic cancer. Oncogene, 38(18), 3355-3370.
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2

Cell Viability and Proliferation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Treatment responses were measured using crystal violet staining or ELISA BrdU incorporation (Sigma 11669915001) as described by manufacturer. Cell Titer Glo was used to measure ATP levels and CyQuant was used to measure DNA content in treated cultures. Luminescence/fluorescence was read on a BioTek Synergy 2 plate reader. Live cell analysis was performed using 96-well collagen-coated tissue culture treated plates in IncuCyte S3 Live-Cell Analysis System (Essen Biosciences). Essen Bioscience software was used to quantify number of H2B-GFP positive cells per well and then normalized to starting cell number for each condition. Data was exported to Prism 7 (GraphPad) for statistical analysis and graph generation. Each drug treatment was carried out with multiples greater than six and verified in two independent experiments.
Knudsen E.S., Kumarasamy V., Ruiz A., Sivinski J., Chung S., Grant A., Vail P., Chauhan S.S., Jie T., Riall T.S, & Witkiewicz A.K. (2019). Cell cycle plasticity driven by MTOR signaling: Integral resistance to CDK4/6 inhibition in patient-derived models of pancreatic cancer. Oncogene, 38(18), 3355-3370.
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