Between 2009 and 2013, the University La Paz Hospital Laboratory utilized an Olympus 5400 analyzer (Beckman Coulter) to measure serum ALP activity. In February 2014, it switched to Siemens Healthineers (Advia 2400 chemistry system) and clinically acceptable correlation and comparison was demonstrated between the two devices (internal Laboratory data available). Both methods measure ALP activity by a kinetic rate method in which p-nitrophenyl phosphate (a colourless organic phosphate ester substrate) is hydrolyzed by ALP to the yellow-coloured product pnitrophenol and phosphate at pH 10,3. Enzymatic activity of ALP is directly proportional to changes in absorbance at 410 nm. The normal adult’s range is 45 to 116 IU/L.
Advia 2400 chemistry
Manufactured by Siemens
Sourced in United States
The Advia 2400 is a clinical chemistry analyzer designed for high-volume laboratory testing. It is capable of performing a wide range of clinical chemistry tests, including assays for enzymes, electrolytes, and other analytes. The Advia 2400 is designed to provide accurate and reliable results with a high throughput, making it suitable for use in busy clinical laboratories.
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Lab products found in correlation
2 protocols using advia 2400 chemistry
1
Serum ALP Measurement Transition
2
Blood Collection and Analysis Protocol
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Blood samples were collected by registered nurses or registered blood technicians using vacuum plastic tubes (spray dried K3EDTA vacuum tube and serum gel separator). Complete blood count was analyzed using DxH800 Beckman Coulter CBC analyzers (Brea, CA, USA). Wide-range CRP in human serum was analyzed by an immunoturbidimetric assay with the Siemens ADVIA 2400 chemistry system using a dedicated reagent (SIEMENS Healthcare Diagnostics Inc., Tarrytown, NY, USA) [27 (link)].
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