Anti α sma a5228

For histological examination, hematoxylin and eosin (H&E) staining, and Movat pentachrome staining were performed. Anti-CD31 (MAB1398Z; Millipore, Burlington, MA), anti-alpha-smooth muscle actin (anti-α-SMA; A5228; Sigma-Aldrich, St. Louis, MO; and ab5694; Abcam, Cambridge, United Kingdom), anti-CD45 (Mab114; R&D Systems, Minneapolis, MN), anti-PRDX2 (ab109367; Abcam), anti-4-hydroxynonenal (anti-4-HNE; ab46545; Abcam), anti-DNA/RNA damage antibody (anti-8-OHG) (ab62623; Abcam), and anti-mouse macrophage/monocyte antibody (anti-MOMA2) (MCA519G; Bio-Rad, Hercules, CA) were used as primary antibodies for immunostaining. After incubation with the primary antibodies, Alexa 488 and 594 (Invitrogen, Carlsbad, CA) or biotinylated secondary antibodies with 3,3′-diaminobenzidine substrate (Vector Laboratories, Burlingame, CA) were used to visualize the antigens. Furthermore, 4′,6-diamidino-2-phenylindole (DAPI) or hematoxylin was used to label the nuclei. The negative control tissues were prepared in a similar manner using IgG isotype control antibodies (Santa Cruz Biotechnology, Dallas, TX). The immunofluorescence was imaged with an LSM 510 meta confocal microscope (Carl Zeiss, Oberkochen, Germany) or a BX53 microscope (Olympus, Tokyo, Japan).

The primary antibodies used in this study include anti-Fibronectin (ab19245) and anti-PARK7/DJ 1(ab18257) from Abcam, anti-cleaved caspase3 (9664), anti-α/β-Tubulin(2148) and anti-GAPDH (5174) from Cell Signaling Technology, anti-Lamin B1 (66095-1-Ig) and anti-Myc tag (6003-2-Ig) from Proteintech, anti-Collagen I (AF7001) from Affinity, anti-α-SMA(A5228) from Sigma-Aldrich, anti-SOD2(sc-137254) from Santa Cruz, and anti‐F4/80 (GB11027) from Servicebio. All secondary antibodies were purchased from Thermo‐Fisher Scientific. Special reagents were from the following sources: CM-H2DCFDA(C6827), Mito-SOX (M36008) and DHE (D11347) from Thermo Fisher Scientific, TUNEL assay kit (12156792910) from Roche Life Science, and recombinant human TGFB1 (GF111) from EMD Millipore. The PARK7-based peptide ND-13 (YGRKKRRKGAEEMETVIPVD) was synthesized by China Peptides (China).