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Alexafluor 647 donkey anti goat igg a21447

Manufactured by Thermo Fisher Scientific

AlexaFluor 647 donkey anti-goat IgG (A21447) is a secondary antibody conjugate used for immunofluorescence detection. It is designed to bind to goat primary antibodies and is labeled with the AlexaFluor 647 fluorescent dye.

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2 protocols using alexafluor 647 donkey anti goat igg a21447

1

Confocal Imaging of Fluorescently Labeled Cells

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Microscope images were taken using a Zeiss axiovert 200M confocal microscope equipped with LSM 510 3 channel confocal imaging system, and a Zeiss 63×/1.4 DIC Plan-Apochromat, oil immersion objective lens at 25°C. Cells were stained using AlexaFluor 488 donkey anti-mouse IgG (A21202), AlexaFluor 546 donkey anti-rabbit (A10040), and AlexaFluor 647 donkey anti-goat IgG (A21447) (Invitrogen). Images were acquired using Zeiss LSM 510 ver3.2 software, and processed using Image J to colour split/merge channels.
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2

Confocal Imaging of Fluorescently Labeled Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microscope images were taken using a Zeiss axiovert 200M confocal microscope equipped with LSM 510 3 channel confocal imaging system, and a Zeiss 63×/1.4 DIC Plan-Apochromat, oil immersion objective lens at 25°C. Cells were stained using AlexaFluor 488 donkey anti-mouse IgG (A21202), AlexaFluor 546 donkey anti-rabbit (A10040), and AlexaFluor 647 donkey anti-goat IgG (A21447) (Invitrogen). Images were acquired using Zeiss LSM 510 ver3.2 software, and processed using Image J to colour split/merge channels.
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