Escherichia coli dh5 αz1

Escherichia coli DH5 αZ1 (Invitrogen) was used for subcloning and conjugation. Escherichia coli cells were grown in LB medium at 37 °C and supplemented with appropriate antibiotics to the final concentrations in the medium: 50 μg ml⁻¹ kanamycin (Km) or/and 20 μg ml⁻¹ chloramphenicol (Cm) (Sigma, Merk).
Synechocystis sp. PCC 6803, a unicellular glucose-tolerant strain was used in this study. Cultures were grown in BG11 medium [45 (link)] with respective antibiotics Km 25 μg ml⁻¹ and/or Cm 20 μg ml⁻¹ at 30 °C under constant light. The optical density of Synechocystis cultures at 750 nm was measured using a Varian Cary 50 BIO spectrophotometer, and an OD₇₅₀ value of 1 corresponds to approximately 10⁸ cells.

Escherichia coli DH5α-Z1 (Invitrogen) was used for subcloning and conjugation. E. coli cultures were cultivated in LB medium at 37 °C and supplemented with appropriate antibiotics to the final concentrations in the medium: 50 μg·ml⁻¹ kanamycin (Km) or 200 μg·ml⁻¹ erythromycin (Em) (Sigma, Merck).
In this study the model cyanobacterial strain Synechocystis sp. PCC 6803, a glucose-tolerant unicellular strain, was used. Cultures were grown in BG11 medium [32 (link)] with respective antibiotics Km 25 μg·ml⁻¹ and/or Em 25 μg·ml⁻¹ at 30 °C under constant 45 µmol photons m⁻² s⁻¹ light. The optical density of Synechocystis cultures was measured on Varian Cary 50 BIO spectrophotometer at a wavelength of 750 nm.