Cellcheck

Manufactured by IDEXX

Sourced in United States

CellCheck is a diagnostic tool used by veterinary professionals to analyze cell samples. It provides precise cell counts and differentials, enabling accurate assessment of the health and function of cells. CellCheck is designed to support veterinary diagnostics and decision-making processes.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

CellCheck Mouse Plus CellCheck mouse 19 CellCheck 9 Plus CellCheck Cell Line Authentication CellCheck 16 – human CellCheck 19 plus CellCheck short tandem repeat profiling service

25 protocols using cellcheck

Bovine Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Madin-Darby bovine kidney (MDBK) cells were obtained from the American Type Cell Culture Collection (CCL-22^TM). Cells were confirmed by an independent laboratory to be of bovine origin without Mycoplasma or mammalian interspecies contamination (CellCheck^TM and STAT-Myco^TM, IDEXX Laboratories, Inc., Westbrook, ME, United States). Culture medium consisted of minimum essential medium supplemented with Earle’s salts and 10% equine serum, sodium bicarbonate (0.7 μg/ml), and penicillin (100 U/ml).

Kuca T., Passler T., Newcomer B.W., Neill J.D., Galik P.K., Riddell K.P., Zhang Y., Bayles D.O, & Walz P.H. (2020). Changes Introduced in the Open Reading Frame of Bovine Viral Diarrhea Virus During Serial Infection of Pregnant Swine. Frontiers in Microbiology, 11, 1138.

+ Open protocol

+ Expand

Bovine Kidney Cell Cultivation and Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Madin-Darby bovine kidney cells were purchased from the American Type Cell Culture Collection (CCL-22^TM) and confirmed to be of bovine origin through amplification and sequencing of mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (CellCheck^TM, IDEXX Laboratories, Inc., Westbrook, ME, United States). PCR assays also demonstrated the absence of Mycoplasma and mammalian interspecies contamination (STAT-Myco^TM, IDEXX Laboratories, Inc.). Cells were grown in minimum essential medium (MEM) with Earle’s salts supplemented with 10% equine serum, L-glutamine (0.02 mM), sodium bicarbonate (0.75 mg/ml), penicillin (100 U/ml), and streptomycin (100 μg/ml).

Kuca T., Passler T., Newcomer B.W., Neill J.D., Galik P.K., Riddell K.P., Zhang Y, & Walz P.H. (2018). Identification of Conserved Amino Acid Substitutions During Serial Infection of Pregnant Cattle and Sheep With Bovine Viral Diarrhea Virus. Frontiers in Microbiology, 9, 1109.

+ Open protocol

+ Expand

Authentication and Culture of HeyA8 OvCa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HeyA8 OvCa cell lines were provided by Dr. Gordon Mills. Cell lines were authenticated using CellCheck (IDEXX Bioresearch). HeyA8 cells were first cultured in 10 cm dish with full growth medium containing RPMI 1640, 10 % FBS, 100 U/ml penicillin and 100 µg/ml streptomycin.

Liu X., Romero I.L., Litchfield L.M., Lengyel E, & Locasale J.W. (2016). Metformin targets central carbon metabolism and reveals mitochondrial requirements in human cancers. Cell metabolism, 24(5), 728-739.

+ Open protocol

+ Expand

Cell Line Authentication and Cultivation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were tested for mycoplasma and authenticated using a commercial service (CellCheck, IDEXX Bioresearch). The ovarian cancer cell lines SKOV3ip1, OVCAR5, and COV318 were cultured in DMEM + 10% FCS + 1% pen/strep and are of female origin. 59M (ovarian cancer) was cultured in DMEM + 10% FCS + 1% pen/strep + 10μg/mL insulin and is of female origin. HCC1143 (breast cancer) and OVKATE (ovarian cancer) were cultured in RPMI 1640 + 10% FCS + 1% pen/strep and are of female origin. MFM223 (breast cancer) was cultured in MEM + 10% FCS + 1% pen/strep and is of female origin. JY (lymphoblastoid) was cultured in RPMI 1640 + 10% FCS + 1% pen/strep and is of male origin. Derivatives of these cell lines generated in this paper were maintained under the same conditions. A complete list of cell lines can be found in the KEY RESOURCES TABLE.

Coscia F., Lengyel E., Duraiswamy J., Ashcroft B., Bassani-Sternberg M., Wierer M., Johnson A., Wroblewski K., Montag A., Yamada S.D., López-Méndez B., Nilsson J., Mund A., Mann M, & Curtis M. (2018). Multi-level Proteomics Identifies CT45 as a Chemosensitivity Mediator and Immunotherapy Target in Ovarian Cancer. Cell, 175(1), 159-170.e16.

+ Open protocol

+ Expand

Characterization of ovarian cancer cell lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKOV3ip1 and HeyA8 cells were provided by Dr. Gordon Mills (M.D. Anderson Cancer Center, Houston, TX). HeyA8-MDR was a gift from Dr. Anil Sood (M.D. Anderson Cancer Center, Houston, TX) (30 (link)). SKOV3-Taxol resistant cells (TR) were provided by Dr. Zhenfeng Duan (MGH, Boston MA) (31 (link)). Tyk-nu cells were provided by Dr. Kenjiro Sawada (Osaka University, Japan) (32 (link)). The Tyk-nu carboplatin-resistant (R) cell line (33 (link)) was purchased from JCRB cell bank. Tyk-nu and Tyn-nu-R cells were cultured in Eagle’s Minimum Essential Medium with 15% fetal bovine serum, 1% penicillin–streptomycin and 1% L-glutamine (34 (link)). All other human OvCa cell lines were maintained in Dulbecco’s modified Eagle’s medium with 10% FBS and 1% penicillin-streptomycin solution at 37°C with 5% CO₂. All cell lines were authenticated using the commercial service, CellCheck (IDEXX Bioresearch). Samples were confirmed to be of human origin and no mammalian inter-species contamination was detected. The alleles for 9 short tandem repeat (STR) markers were determined and the results were compared to the profiles from DSMZ, ATCC, JCRB, and RIKEN STR databases.

Zhang Y., Sriraman S.K., Kenny H.A., Luther E., Torchilin V, & Lengyel E. (2016). Reversal of chemoresistance in ovarian cancer by co-delivery of a P-glycoprotein inhibitor and paclitaxel in a liposomal platform. Molecular cancer therapeutics, 15(10), 2282-2293.

+ Open protocol

+ Expand

Establishment and Characterization of PDAC Cell Lines

Cited 4 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Low-passage patient-derived PDAC cell lines (fewer than 35 passages from patient tumors) and pancreatic cancer-associated fibroblasts used in this project were received from Dr. Anirban Maitra (The Johns Hopkins University) and maintained as previously described^{15 (link),22 (link),38 (link),39 (link)}. Cells were submitted for STR analysis (CellCheck with IDEXX BioResearch) and were regularly confirmed to be free of mycoplasma contamination. Cell lines were passaged fewer than 12 times before resuscitating fresh stocks. Hypoxic conditions in monolayer experiments were generated in a Ruskinn Invivo₂ 200 hypoxia work station (Baker Ruskinn; Sanford, ME) at 0.2% oxygen. Cell proliferation and viability in monolayer cultures was measured with alamarBlue assay as previously described^{15 (link),39 (link)}. Growth of 3-dimensional tumor spheroid cultures was performed and quantified as described previously using tumor cells stably transduced with TdTomato (red), and CAFs stably transduced with EGFP (green) to differentiate the two cell types in 3D co-cultures and track the growth of each over time^{15 (link),40 (link),41 (link)}.

Logsdon D.P., Shah F., Carta F., Supuran C.T., Kamocka M., Jacobsen M.H., Sandusky G.E., Kelley M.R, & Fishel M.L. (2018). Blocking HIF signaling via novel inhibitors of CA9 and APE1/Ref-1 dramatically affects pancreatic cancer cell survival. Scientific Reports, 8, 13759.

+ Open protocol

+ Expand

Established and Patient-Derived PDAC Cell Culture

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Established PDAC cell lines, BxPC-3 and Panc-1, were obtained from American Type Culture Collection (Manassas, VA) and were grown in RPMI-1640 (Life Technologies, Grand Island, NY) and high glucose DMEM (Hyclone, Logan, UT), respectively. Low-passage patient-derived PDAC cells, 10.05, as well as CAFs were grown in high glucose DMEM without sodium pyruvate (Life Technologies)^{54 (link),87 (link)}. All medium was supplemented with 10% heat-inactivated fetal bovine serum (HI FBS; Life Technologies). Medium for BxPC-3 and Panc-1 was also supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Sigma Aldrich, St. Louis, MO), while 10.05 and CAFs were cultured in absence of antibiotics. Cells were maintained in a humidified environment of 5% CO₂ in air at 37 °C. All cells were passaged at 70–90% confluency; established PDAC lines and CAFs were used below passage 20. Patient-derived PDAC cells were used below passage 10, were authenticated by STR analysis (CellCheck with IDEXX BioResearch) and were tested regularly for mycoplasma contamination.

Puls T.J., Tan X., Husain M., Whittington C.F., Fishel M.L, & Voytik-Harbin S.L. (2018). Development of a Novel 3D Tumor-tissue Invasion Model for High-throughput, High-content Phenotypic Drug Screening. Scientific Reports, 8, 13039.

+ Open protocol

+ Expand

Cell Line Maintenance and Authentication

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

NIH/3T3 (ATCC^® CRL1658™), 293T (ATCC^® CRL-3216^™) and HCT116 (ATCC^® CCL-247^™) cells were maintained in DMEM medium supplemented with 10% fetal bovine serum (FBS) with antibiotics at 37°C in a humidified atmosphere containing 5% CO₂ and 95% O₂. Cell lines were authenticated using CellCheck by IDEXX Bioresearch, and tested for mycoplasma using PlasmoTest™ - Mycoplasma Detection Kit (InvivoGen, Inc). Hras^-/-;Nras^-/-;Kras^lox/lox;RERT^ert/ert MEFs were kindly provided by Mariano Barbacid (Spanish National Cancer Research Centre) and were maintained as described previously [17 (link)]. Immortalized Sirt2^+/+ and Sirt2^-/- MEFs were made as described previously [5 (link)]. Cell lines were cultured for less than 20 passages and frozen down from the first 3 passages for further experiments.

Song H.Y., Biancucci M., Kang H.J., O'Callaghan C., Park S.H., Principe D.R., Jiang H., Yan Y., Satchell K.F., Raparia K., Gius D, & Vassilopoulos A. (2016). SIRT2 deletion enhances KRAS-induced tumorigenesis in vivo by regulating K147 acetylation status. Oncotarget, 7(49), 80336-80349.

+ Open protocol

+ Expand

Comprehensive Characterization of Lymphoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were cultured according to ATCC-recommended culture conditions. Passage numbers were limited to <20 passages from the original culture obtained from vendors. Ramos Burkitt's lymphoma cell line (ATCC, catalog no. CRL-1596, RRID: CVCL_0597) and REC-1 mantle cell lymphoma (ATCC, catalog no. CRL-3004, RRID:CVCL_1884) were obtained from ATCC. TMD8 DLBCL cell line (RRID: CVCL_A442) was licensed and procured from Tokyo Medical and Dental University in Japan and used for in vitro assays. All cell lines used in this study were authenticated using short tandem repeat DNA profiling (CellCheck, IDEXX BioAnalytics). Cell line identity and Mycoplasma testing using real-time PCR (STAT-Myco, IDEXX BioAnalytics) were last conducted on the Ramos Burkitt's lymphoma cell line on October 9, 2020, and on the REC-1 mantle cell and TMD8 DLBCL cell lines on October 19, 2020. The TMD8 and the REC-1 MCL xenograft models were used to assess in vivo activities of compounds. BTK, RAC1, and RAC2 knockouts or knockdowns were generated in Ramos and TMD8 cells using CRISPR technology (Supplementary Methods).

Li W., Sano R., Apatira M., DeAnda F., Gururaja T., Yang M., Lundgaard G., Pan C., Liu J., Zhai Y., Yoon W.H., Wang L., Tse C., Souers A.J, & Lee C.H. (2023). Bruton's Tyrosine Kinase Inhibitors with Distinct Binding Modes Reveal Differential Functional Impact on B-Cell Receptor Signaling. Molecular Cancer Therapeutics, 23(1), 35-46.

+ Open protocol

+ Expand

Culturing Human Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

LNCaP [45 (link)] (ATCC, Manassas, VA) and PC-3M [46 (link)] (a gift from Paula Foster, Western University, London, ON) human PCa cells were maintained in RPMI-1640+10% fetal bovine serum (FBS). LNCaP C4-2B [47 (link)] [C4-2B] human PCa cells (a gift from Katherine Stemke Hale, M.D. Anderson, Houston, TX) were maintained in T-media+10% FBS. PC-3 human PCa cells [48 (link)] (ATCC) were maintained in F12K media+10%FBS. MDA-MB-468 human breast cancer cells [49 (link)] (a gift from Janet Price, M.D. Anderson) were maintained in αMEM+10%FBS. HeLa human cervical cancer cells [50 (link)] (a gift from Jim Koropatnick, Western University) were maintained in DMEM+10%FBS. Media/reagents and FBS were obtained from Life Technologies (Carlsbad, CA) and Sigma (St. Louis, MO), respectively. Cell lines were authenticated via third-party testing (CellCheck, IDEXX BioResearch, Columbia, MO) in December 2015.

Lowes L.E., Goodale D., Xia Y., Postenka C., Piaseczny M.M., Paczkowski F, & Allan A.L. (2016). Epithelial-to-mesenchymal transition leads to disease-stage differences in circulating tumor cell detection and metastasis in pre-clinical models of prostate cancer. Oncotarget, 7(46), 76125-76139.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!