Log In Sign up
The largest database of trusted experimental protocols

N 6705

Manufactured by Merck Group
Visit Supplier

N-6705 is a laboratory instrument designed for conducting various analytical and scientific experiments. It is a versatile piece of equipment that can be used in a wide range of applications. The core function of N-6705 is to provide a controlled environment for conducting experiments and analyzing samples. It is a reliable and accurate tool for researchers and scientists in various fields.

Automatically generated - may contain errors

Lab products found in correlation

N 5755, by Merck Group (2 mentions) Nadp nadph glo assay, by Promega (2 mentions) Fluostar omega, by BMG Labtech (2 mentions)

2 protocols using n 6705

1

Quantifying NADPH/NADP+ in Melanoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous tumors were surgically excised as quickly as possible after killing the mice then melanoma cells were mechanically dissociated and NADPH and NADP+ were measured using the NADPH/NADP Glo-Assay (Promega) following the manufactures instructions. Standard curves were generated using purified NADP+ (N-5755, Sigma-Aldrich) and NADPH (N-6705, Sigma-Aldrich) prepared in the same buffers used for the experimental samples. The absolute amounts of NADP+ and NADPH in each sample was then determined using these standard curves. Luminescence was measured using a using a FLUOstar Omega plate reader (BMG Labtech). Values were normalized to tissue mass.
Tasdogan A., Faubert B., Ramesh V., Ubellacker J.M., Shen B., Solmonson A., Murphy M.M., Gu Z., Gu W., Martin M., Kasitinon S.Y., Vandergriff T., Mathews T.P., Zhao Z., Schadendorf D., DeBerardinis R.J, & Morrison S.J. (2019). Metabolic heterogeneity confers differences in melanoma metastatic potential. Nature, 577(7788), 115-120.
+ Open protocol
+ Expand
2

Quantifying NADPH/NADP+ in Melanoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous tumors were surgically excised as quickly as possible after killing the mice then melanoma cells were mechanically dissociated and NADPH and NADP+ were measured using the NADPH/NADP Glo-Assay (Promega) following the manufactures instructions. Standard curves were generated using purified NADP+ (N-5755, Sigma-Aldrich) and NADPH (N-6705, Sigma-Aldrich) prepared in the same buffers used for the experimental samples. The absolute amounts of NADP+ and NADPH in each sample was then determined using these standard curves. Luminescence was measured using a using a FLUOstar Omega plate reader (BMG Labtech). Values were normalized to tissue mass.
Tasdogan A., Faubert B., Ramesh V., Ubellacker J.M., Shen B., Solmonson A., Murphy M.M., Gu Z., Gu W., Martin M., Kasitinon S.Y., Vandergriff T., Mathews T.P., Zhao Z., Schadendorf D., DeBerardinis R.J, & Morrison S.J. (2019). Metabolic heterogeneity confers differences in melanoma metastatic potential. Nature, 577(7788), 115-120.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!