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2 protocols using anti p21 antibody k0081 3

1

Antibody Immunodetection Reagents

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Anti-p53 antibody (DO-1; sc-126), HRP-conjugated anti-rat antibody (sc-2032), and AP-conjugated anti-rat antibody (sc-2021) were obtained from Santa Cruz Biotechnology; HRP-conjugated anti-rabbit antibody (W4011), HRP-conjugated anti-mouse antibody (W4021), and AP-conjugated anti-mouse antibody (S3721) were from Promega; anti-α-tubulin antibody (T9026) and anti-γ-tubulin antibody (T6557) were from Sigma-Aldrich; anti-phospho-p53 (Ser15) antibody (#9284) and anti-53BP1 antibody (#4937) were from Cell Signaling Technology; anti-DAO antibody (6844-1) was from Abcam; anti-HA antibody (1867423) was from Roche; anti-p21 antibody (K0081-3) was from Medical and Biological Laboratories; and AP-conjugated anti-rabbit antibody (59298) was from MP Biomedicals.
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2

Protein Expression Analysis by Western Blot

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Anti-Caspase-3 antibody (#9662), anti-PARP antibody (#9542), anti-phospho-Chk1 (Ser296) antibody (#2349), anti-phospho-Chk1 (Ser317) antibody (#2344), anti-phospho-Chk1 (Ser345) antibody (#2348), anti-Chk1 antibody (#2345), anti-phospho-Chk2 (Thr68) antibody (#2661), anti-phospho-Chk2 (Ser516) antibody (#2669), anti-Chk2 antibody (#2662), anti-phospho-p53 (Ser15) antibody (#9284), and anti-phospho-p53 (Ser20) antibody (#9287) were obtained from Cell Signaling Technology; anti-p53 antibody (DO-1; sc-126), anti-PRODH antibody (sc-376401), and HRP-conjugated anti-rat antibody (sc-2032) were from Santa Cruz Biotechnology; anti-α-tubulin antibody (T9026) and anti-γ-tubulin antibody (T6557) were from Sigma Aldrich; anti-p21 antibody (K0081-3) and anti-ATM antibody (PM026) were from Medical and Biological Laboratories; HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were from Promega; anti-HA antibody (1867423) was from Roche; anti-DAO antibody (6844-1) was from Abcam.
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