Anti β tubulin

Manufactured by Novus Biologicals

Anti-β-Tubulin is a primary antibody that binds to the beta subunit of tubulin, a structural protein found in eukaryotic cells. It is commonly used in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and visualize the presence and distribution of beta-tubulin in biological samples.

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Lab products found in correlation

Nb100 2220, by Novus Biologicals (1 mentions) Irdye 680lt goat anti mouse, by LI COR (1 mentions) Anti sqstm1, by BD (1 mentions) Fluorescently conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Nanozoomer 2.0 rs scanner, by Hamamatsu Photonics (1 mentions) Zen digital imaging for lsm 700, by Zeiss (1 mentions) Zen 2010b sp1 imaging software, by Zeiss (1 mentions) Nanozoomer digital pathology software, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

Anti-β-tubulin-647 antibody β-tubulin

2 protocols using anti β tubulin

Multifaceted Western Blot Analysis

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A combination of rabbit polyclonal anti-MAP1LC3B (Novus, NB100-2220) at 1:5000 and mouse monoclonal anti-β-Tubulin (DSHB, E7) at 1:2000 dilution was used for Western blotting of chloroquine treated cell lysates.
A combination of rabbit polyclonal anti-HDAC2 (Thermo Scientific, PA1-861) at 1:3000 and mouse monoclonal anti-β-Tubulin (DSHB, E7) at 1:2000 was used as positive controls for the nuclear and cytoplasmic fractions, respectively. A combination of mouse monoclonal anti-GFP (Clontech, 632381) at 1:2000 and rabbit polyclonal anti-MAP1LC3B (Novus, NB100-2220) at 1:5000 was used to analyze the IP fractions. For validation of the MudPIT results, Western blots were probed with either mouse monoclonal anti-SQSTM1 (BD, 610832) at 1:2000 dilution or goat polyclonal anti-MAP1B (Santa Cruz, sc-8970) at 1:100 dilution in combination with rabbit polyclonal anti-MAP1LC3B (Novus, NB100-2220) at 1:5000.
Secondary antibodies used for Western blotting included goat anti-rabbit IRDye-800CW (LI-COR, 926-32211), goat anti mouse IRDye 680 LT (LI-COR, 926-68020), donkey anti-goat IRDye-800CW (926-32214), donkey anti-rabbit IRDye 680 LT (926-68023).

Kraft L.J., Manral P., Dowler J, & Kenworthy A.K. (2016). Nuclear LC3 associates with slowly diffusing complexes that survey the nucleolus. Traffic (Copenhagen, Denmark), 17(4), 369-399.

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Immunofluorescence Analysis of PrP Aggregates

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Cells were fixed in 4% paraformaldehyde and 4% sucrose and permeabilized or not for 10 min with 0.2% Triton X-100. After being blocked for 30 min in PBS containing 3% bovine serum albumin and 0.3 M glycine, the cells were incubated overnight with anti-PrP SAF83 antibody (Cayman; 1:2000 dilution) or Sha31 (Spi-Bio) and anti-β-tubulin (Novus Biologicals; 1:2000 dilution), followed by incubation with fluorescently conjugated secondary antibodies (Invitrogen). For the resistance to denaturation, before incubation with antibodies, the cells were treated for 2 h at 4 °C with 3 M guanidine thiocyanate. Cells were scanned with a NanoZoomer 2.0RS scanner and analyzed using NanoZoomer digital pathology software (Hamamatsu Photonics). For confocal analyses, cells were plated on poly-d-lysine (Sigma)-coated microscope cover glasses (Thermo Fisher Scientific). Cell images were acquired with the laser scanning confocal microscope, ZEN Digital Imaging for LSM 700 (Zeiss), and analyzed using Zen 2010b SP1 imaging software (Zeiss) and ImageJ (https://imagej.nih.gov/ij/). For measuring PrP signals, an identical threshold was applied to all images. Then intensities of PrP signals and clustered particles were measured using ImageJ software.

Daude N., Lau A., Vanni I., Kang S.G., Castle A.R., Wohlgemuth S., Dorosh L., Wille H., Stepanova M, & Westaway D. (2022). Prion protein with a mutant N-terminal octarepeat region undergoes cobalamin-dependent assembly into high–molecular weight complexes. The Journal of Biological Chemistry, 298(4), 101770.

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