Peptide purification was carried out using a Shimadzu LC-40 HPLC system equipped with an LC-40D solvent delivery module, photodiode array detector SPD-M40, and degassing unit DGU-403. The sample was dissolved in water and acetonitrile, applied on a Zorbax 300 BS, C18 column (5 mm, 9.4 × 250 mm) from Agilent, and eluted at 1.5 mL/min with a 40 min gradient from 10% to 95% acetonitrile in water (with 0.05% TFA). Preparative injections were monitored at 190, 220, and 254 nm. All products were purified by HPLC to reach a purity of >90%. Peptide synthesis was confirmed using electrospray ionization mass spectrometry (ESI-MS) via the Micromass Quattro Ultima mass spectrometer. ESI-MS and HPLC spectra can be found in the Supporting Information , Figure S8 .
Lc 40d solvent delivery module
Manufactured by Shimadzu
Sourced in Japan
The LC-40D solvent delivery module is a core component of Shimadzu's liquid chromatography systems. Its primary function is to precisely and reliably deliver solvents to the chromatographic system, ensuring accurate and reproducible separation of analytes.
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Lab products found in correlation
Lc 40 hplc system, by Shimadzu (1 mentions)
Frc 10a fraction collector, by Shimadzu (1 mentions)
Vnmrs 600 mhz ft nmr spectrometers, by Agilent Technologies (1 mentions)
Ft ir 4x spectrophotometer, by Jasco (1 mentions)
Spd m40 photo diode array detector, by Shimadzu (1 mentions)
Cbm 40 system controller, by Shimadzu (1 mentions)
Mercury plus 400 mhz, by Agilent Technologies (1 mentions)
Cto 40 s column oven, by Shimadzu (1 mentions)
Apex 2 spectrometer, by Bruker (1 mentions)
V 650 spectrophotometer, by Jasco (1 mentions)
J 815 cd spectrometer, by Jasco (1 mentions)
P 2000 polarimeter, by Jasco (1 mentions)
2 protocols using lc 40d solvent delivery module
1
HPLC Purification of Synthetic Peptides
2
Advanced Analytical Techniques for Compound Characterization
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The Varian (Palo Alto, CA, USA) Mercury Plus 400 MHz and VNMRS 600 MHz FT-NMR spectrometers provided the NMR spectra. HRSIMS was performed by using a Bruker APEX II spectrometer (Bremen, Germany). The V-650 spectrophotometer from Jasco (Tokyo, Japan) was utilized to measure the UV data. For measuring infrared data, the Jasco FT/IR-4X spectrophotometer was selected. Circular dichroism data were recorded using a Jasco J-815 CD spectrometer. A Jasco P-2000 polarimeter was used to quantify specific optical rotation. Merck KGaA (Darmstadt, Germany) silica gel 60 (0.015–0.040 mm) was utilized to pack columns. For high-performance liquid chromatography (HPLC), Phenomenex (Torrance, CA, USA) C18, phenyl-hexyl, and biphenyl columns were utilized. The LC-40D solvent delivery module, DGU-405 de-gassing unit, CBM-40 system controller, CTO-40S column oven, SPD-M40 photo diode array detector, and FRC-10A fraction collector made up the Shimadzu (Kyoto, Japan) HPLC apparatus.
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