To prepare samples for STEM measurements, a thin layer of poly(methyl methacrylate) (PMMA) was deposited onto a SiO2/Si substrate containing MoS2 monolayer crystals using a spin coater (500 rpm for 10 s followed by 3000 rpm for 50 s) and then left in a fume hood overnight to evaporate the solvent. The substrate with PMMA-coated monolayers was dissolved by placing into a petri dish filled with KOH solution (30 weight % at 90°C) leaving a floating PMMA/MoS2 membrane. The membrane was transferred onto a QUANTIFOIL holey carbon TEM grid and then washed in acetone and isopropyl alcohol to remove the PMMA. The TEM grids were baked at 160°C overnight in vacuum before characterization. The atomic resolution HAADF-STEM characterization was conducted using a Nion UltraSTEM100 equipped with a fifth-order aberration corrector and operated at 60 kV. Lateral heterostructure samples were baked overnight at 160°C to mitigate hydrocarbon contamination for image acquisition. The probe current was ~20 pA, and convergence angle was set at 30-mrad semiangle.
Holey carbon tem grid
Manufactured by Quantifoil
Holey carbon TEM grid is a specimen support used in transmission electron microscopy (TEM). It consists of a thin carbon film with an array of holes suspended on a metal grid. The holey carbon film provides a support for the sample being imaged, allowing the electron beam to pass through the holes and interact with the specimen.
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Lab products found in correlation
2 protocols using holey carbon tem grid
1
PMMA-Assisted TEM Sample Preparation for MoS2
2
Vitrification of Lysozyme Nanocrystals
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A TFS Vitrobot
Mark IV was used for vitrification of the lysozyme sample. Around
4 μL of the sample solution with lysozyme nanocrystals was applied
onto a glow-discharged Quantifoil holey carbon TEM grid at 4 °C
and 100% relative humidity. The grid was then plunged frozen in liquid
ethane and mounted onto a Gatan 626 cryotransfer holder for cryoEM
experiments.
Mark IV was used for vitrification of the lysozyme sample. Around
4 μL of the sample solution with lysozyme nanocrystals was applied
onto a glow-discharged Quantifoil holey carbon TEM grid at 4 °C
and 100% relative humidity. The grid was then plunged frozen in liquid
ethane and mounted onto a Gatan 626 cryotransfer holder for cryoEM
experiments.
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