Cells were seeded at 4 × 104 cells/ml in 96 well plates. Adhered cells were incubated in the dark with DCFH-DA to 2′,7′-dichlorofluorescein (1 h/37°C; 40 μM, ThermoFisher Sci). Cells were washed and subjected to different drug treatments for 2h. Fluorescence was analyzed in a plate reader (PHERAstar FS, BMG LABTECH) with excitation at 485 nm and emission at 520 nm.
Dcfh da to 2 7 dichlorofluorescein
Manufactured by Thermo Fisher Scientific
DCFH-DA to 2',7'-dichlorofluorescein is a fluorescent dye used for the detection and measurement of reactive oxygen species in biological systems. It can be used to quantify the level of oxidative stress in cells.
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2 protocols using dcfh da to 2 7 dichlorofluorescein
1
Measuring Cellular Oxidative Stress
2
Intracellular ROS Measurement Assay
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Cells were seeded at 4 × 104 cells/ml in 96 well plates. Adhered cells were incubated in the dark with DCFH-DA to 2′,7′-dichlorofluorescein (1 h/37°C; 40 μM, ThermoFisher Sci). Cells were washed and subjected to different drug treatments for 2h. Fluorescence was analyzed in a plate reader (PHERAstar FS, BMG LABTECH) with excitation at 485 nm and emission at 520 nm
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