Dapi staining

Manufactured by Wuhan Servicebio Technology

Sourced in China

DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye that binds to DNA, specifically to the adenine-thymine (A-T) base pairs. It is commonly used in fluorescence microscopy and flow cytometry applications to stain and visualize cell nuclei.

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Lab products found in correlation

Fluorescence microscope, by Nikon (1 mentions) Fluorescence microscope, by Olympus (1 mentions) Sc 101498, by Santa Cruz Biotechnology (1 mentions) Ab32500, by Abcam (1 mentions) Ab74272, by Abcam (1 mentions) Ethyl acetate, by Sinopharm (1 mentions) Cell counting kit 8 (cck8), by Bioss Antibodies (1 mentions) Methacrylic anhydride, by Maclin Biochemical Technology (1 mentions) ε caprolactone, by Maclin Biochemical Technology (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Gelatin, by Merck Group (1 mentions) Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions) Polyethylene glycol, by Maclin Biochemical Technology (1 mentions) Acetone, by Sinopharm (1 mentions) Petroleum ether, by Sinopharm (1 mentions) Live dead staining kit, by Keygen Biotech (1 mentions) Tritc phalloidin, by Yeasen (1 mentions) Dichloromethane, by Sinopharm (1 mentions)

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DAPI staining solution (5 citations) DAPI staining reagent (3 citations)

4 protocols using dapi staining

Histological and Immunofluorescence Analysis of Adipose and Liver Tissue

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Liver, epididymal adipose, and interscapular brown adipose tissues were fixed in 4% (v/v) paraformaldehyde before being embedded in paraffin. The tissue was sectioned into 4 to 10 µm sections and stained with hematoxylin-eosin (H&E). Oil-Red-O staining was performed to observe liver intracellular lipid accumulation. An optical microscope was used to observe the sections.
IF staining starts with antigen repair. Subsequently, the colon sections were incubated with primary antibodies overnight at 4 °C, then covered with the corresponding secondary antibody and kept at room temperature for 50 min without light. Cell nuclei were counterstained with DAPI staining (Wuhan, China, Servicebio) and analyzed under a fluorescence microscope (Japan, Nikon).

Sheng X., Wang L., Zhan P., He W., Tian H, & Liu J. (2023). Thyme (Thymus quinquecostatus Celak) Polyphenol-Rich Extract (TPE) Alleviates HFD-Induced Liver Injury in Mice by Inactivating the TLR4/NF-κB Signaling Pathway through the Gut–Liver Axis. Foods, 12(16), 3074.

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EDU Assay for Cell Proliferation

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Cell proliferations were determined by an EdU assay. ADSCs were divided into 6 groups as above. After 48 h of culture, the EdU detection was performed according to the manufacturer’s instructions (C0078S, beyotime). Before the cells were incubated in a shaker for 10 min, 100 μL 0.5% TritonX-100 was added into each well and then rinsed with PBS. The cells were incubated with Edu solution for 30 min, then washed, and dried subsequently. DAPI staining (Servicebio, Wuhan, China) was used to estimate the total cell number. The slides were sealed and photographed under a fluorescence microscope. Three random fields of each sample were observed and photographed under a fluorescence microscope (Olympus, Japan). The number of positive cells was quantified by the software of ImageJ.

Wu J., Yu L., Liu Y., Xiao B., Ye X., Zhao H., Xi Y., Shi Z, & Wang W. (2023). Hypoxia regulates adipose mesenchymal stem cells proliferation, migration, and nucleus pulposus-like differentiation by regulating endoplasmic reticulum stress via the HIF-1α pathway. Journal of Orthopaedic Surgery and Research, 18, 339.

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Immunofluorescence Analysis of Prostate Cancer Cell Markers

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Prostate cancer cells were placed on round slides and fixed with paraformaldehyde (4%). 0.3% Triton X-100 was applied to permeabilize the cell membrane. Bovine serum albumin (3%) was added to block nonspecific binding prior to the staining procedure. The cells were incubated overnight with antibodies against AR (ab74272, Abcam), HMGB1 (66525-1-Ig, Proteintech), STAT3 (ab32500, Abcam) and gankyrin (sc-101498, Santa Cruz Biotechnology) at 4°C in a humidified chamber. The slides were washed in PBS three times and then incubated for 1 hour using fluorescent dye-conjugated secondary antibodies (4412S and 4409S, 1:1000, CST) at room temperature in a light-proof moisture chamber. Nuclei were visualized by DAPI staining (0.1 μg/ml, Servicebio) for 10 min. The slides were then examined and captured using a fluorescence microscope.

Peng G., Wang C., Wang H., Qu M., Dong K., Yu Y., Jiang Y., Gan S, & Gao X. (2023). Gankyrin-mediated interaction between cancer cells and tumor-associated macrophages facilitates prostate cancer progression and androgen deprivation therapy resistance. Oncoimmunology, 12(1), 2173422.

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Bioinspired Gelatin-Based Hydrogel for Bone Tissue Engineering

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Gelatin, from cold‐water fish skin, was purchased from Sigma‐Aldrich (Shanghai, China). Methacrylic anhydride, ε‐caprolactone and polyethylene glycol (PEG) (Mw = 4000) were purchased from Macklin (Shanghai, China). Tin (II) 2‐ethylhexanoate and sodium laurylsulfonate were purchased from Aladdin (Shanghai, China). Ethylacetate, acetone, petroleum ether and dichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Lithium phenyl(2,4,6‐trimethylbenzoyl) phosphinate (LAP) was purchased from Shanghai Yinchang New Material Biological Co., Ltd. Methylamidated OP3 peptide (YCEIEFCYLIR) was purchased from Jiangsu Ji Tai Peptide Industry Science and Technology Co., Ltd. Streptomycin sulfate, penicillin, fetal bovine serum and trypsin were purchased from Gibco (Shanghai, China). Cell counting kit 8 was purchased from Bioss antibodies (Beijing, China). Live/Dead staining kit was purchased from KeyGEN BioTECH (Jiangsu, China). TRITC Phalloidin was purchased from YEASEN (Shanghai, China). Rabbit Anti‐RUNX2 antibody, Alizarin Red S Staining Kit, DAPI staining, and Anti‐Osteocalcin Rabbit pAb were purchased from Servicebio (Wuhan, China). Rat bone marrow mesenchymal stem cells were purchased from ChuangSeed Biomaterials.

Luo P., Fang J., Yang D., Yu L., Chen H., Jiang C., Guo R., Zhu T, & Tang S. (2022). OP3‐4 peptide sustained‐release hydrogel inhibits osteoclast formation and promotes vascularization to promote bone regeneration in a rat femoral defect model. Bioengineering & Translational Medicine, 8(2), e10414.

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