Omnimap anti goat multimer

Necropsies and tissue sampling were performed according to IBC-approved protocols. Tissues were fixed for a minimum of 7 days in 10% neutral buffered formalin with 2 changes. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek, on a 12-hour automated schedule, using a graded series of ethanol, xylene, and ParaPlast Extra. Prior to staining, embedded tissues were sectioned at 5 μm and dried overnight at 42°C. Using GenScript U864YFA140–4/CB2093 NP-1 (1:1000) specific anti-CoV immunoreactivity, CD3 (Predilute) (Roche Tissue Diagnostics #790–4341), and PAX5 (1:500) (Novus Biologicals #NBP2–38790) were detected using the Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) as the secondary antibody. Iba-1 (1:500) (abcam #ab5076) was detected using Roche Tissue Diagnostics OmniMap anti-goat multimer (#760–4647) as the secondary antibody. The tissues were stained using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit Roche Tissue Diagnostics (#760–159).

Necropsies and tissue sampling were performed according to IBC-approved protocols. Tissues were fixed for a minimum of 7 days in 10% neutral buffered formalin with 2 changes. Tissues were placed in cassettes and processed with a Sakura VIP-6 Tissue Tek, on a 12 h automated schedule, using a graded series of ethanol, xylene, and ParaPlast Extra. Prior to staining, embedded tissues were sectioned at 5 µm and dried overnight at 42 °C. Using GenScript U864YFA140-4/CB2093 NP-1 (1:1000) specific anti-CoV immunoreactivity, CD3 (Predilute) (Roche Tissue Diagnostics #790-4341), and PAX5 (1:500) (Novus Biologicals #NBP2-38790, Littleton, CO, USA) were detected using the Vector Laboratories ImPress VR anti-rabbit IgG polymer (# MP-6401) as the secondary antibody. Iba-1 (1:500) (abcam #ab5076) was detected using Roche Tissue Diagnostics OmniMap anti-goat multimer (#760-4647) as the secondary antibody. The tissues were stained using the Discovery Ultra automated stainer (Ventana Medical Systems, Tucson, AZ, USA) with a ChromoMap DAB kit Roche Tissue Diagnostics (#760-159). Histopathology was assessed by a board-certified veterinary pathologist using criteria as previously applied to the Syrian hamster SARS COV-2 model.