For phenotypic analysis of DCs migrating from penile and cervical tissue, a multicolored flow cytometry antibody cocktail was used, consisting of CD14 Qdot605 (TüK4) (Invitrogen), CD11c AF700 (B-ly6), CD123 PECy5 (9F5), CD209 V450 (DCN46), Lin-1 fluorescein isothiocyanate (FITC), CD1c PECy7 (L161) (BioLegend, United Kingdom), HLA-DR allophycocyanin (APC) H7 (G46-6), CD207 APC (10E2) (BioLegend, United Kingdom), and CD83 phycoerythrin (PE) (HB15e). Unless otherwise specified, all antibodies were obtained from BD Biosciences, United Kingdom. Dead cells were excluded from analysis through staining with Aqua viability dye (Invitrogen, United Kingdom). Samples were acquired using an LSRIIFortessa fluorescence-activated cell sorter (FACS; BD Biosciences, United Kingdom) and analyzed using FlowJo (Tree Star, USA). Compensation matrices were created on FlowJo using single stained anti-mouse Ig(κ) negative-control compensation beads (BD Biosciences, United Kingdom).
Cd14 qdot605 t k4
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The CD14 Qdot605 (TüK4) is a fluorescently labeled antibody used in flow cytometry applications. It is designed to detect the CD14 cell surface antigen, which is expressed on various immune cells. The Qdot605 fluorescent label provides a specific emission wavelength for detection and quantification purposes.
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Lab products found in correlation
Aqua viability dye, by Thermo Fisher Scientific (2 mentions)
Lsrii fortessa, by BD (1 mentions)
Cd19 fitc, by Thermo Fisher Scientific (1 mentions)
Cd3 v450 ucht1, by BioLegend (1 mentions)
Cd4 pe cy7 sk3, by BioLegend (1 mentions)
Cd3 v450 ucht1, by Thermo Fisher Scientific (1 mentions)
Cd16 pacific orange 3g8, by Thermo Fisher Scientific (1 mentions)
Cd89 pe a59, by Thermo Fisher Scientific (1 mentions)
Cd16 pecy7 3g8, by BioLegend (1 mentions)
Cd15 bv650 w6d3, by BioLegend (1 mentions)
2 protocols using cd14 qdot605 t k4
1
Multicolor Flow Cytometry for Dendritic Cell Analysis
2
Multicolor Flow Cytometry for FcR Expression
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Red blood cell-lysed whole blood or cells isolated from mucosal tissues were stained using a combination of multicoloured flow cytometry panels designed to determine Fc-receptor expression on CD14+ monocytic cells, mDC or NK cells. Briefly, for overall cellular phenotyping; CD3 V450 [UCHT1], CD4 PECy7 [SK3] (Biolegend), CD8 Pacific Orange [3B5] (Invitrogen), CD19 BV650 [SJ25C1]. For CD14 and mDC FcR Phenotyping; CD3 V450 [UCHT1], CD14 Qdot 605 [TüK4] (Invitrogen), CD16 Pacific Orange [3G8] (Invitrogen), CD11c A700 [B-ly6], CD123 PECy5 [9F5], CD32 APC [FLI8.26], CD64 APC H7 [10.1], CD89 PE [A59], CD19 FITC [HIB19]. For NK cell FcR phenotyping; CD15 BV650 [W6D3] (Biolegend), CD16 PECy7 [3G8] (Biolegend), CD66b FITC [G10F5] (Biolegend), CD64 APC H7 [10.1], CD56 PECy5 [HCD56] (Biolegend), CD45 A700 [HI30] (Biolegend), CD89 PE [A59], CD3 V450 [UCHT1], CD32 APC [FLI8.26]. Unless otherwise specified, all antibodies were sourced from BD Biosciences. Anti-FcRs were able to detect antibody-occupied FcR as indicated by the manufacture and in house controls. Dead cells were excluded from analysis through staining with Aqua Viability Dye (Invitrogen).
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