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Malonaldehyde mda

Manufactured by Nanjing Jiancheng
Sourced in China

Malonaldehyde (MDA) is a small organic compound that is commonly used as a biomarker in biochemical and medical research. It is a reactive species that is formed as a byproduct of lipid peroxidation, a process where free radicals damage cell membranes. MDA can be quantified and analyzed using various laboratory techniques to assess oxidative stress levels in biological samples.

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4 protocols using malonaldehyde mda

1

Curcuminoid Formulations for Biomedical Evaluation

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Curcuminoids, mainly including 82.22% curcumin, 12.43% demethoxycurcumin and 2.44% bisdemethoxycurcumin, was purchased from Nanjing Zelang Biological Technology Co., Ltd (Nanjing, China). The suspension and SEDDS of curcuminoids were prepared with 0.5% sodium carboxyl methyl cellulose (CMC-Na) solution and the reported method (Ke et al., 2014 ). Ethyl carbamate and benzylpenicillin sodium were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) and North China Pharmaceutical Co., Ltd (Shijiazhuang, China), respectively. B-type natriuretic peptide (BNP), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and malonaldehyde (MDA) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
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2

Nanoparticle-based Antioxidant Delivery

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TGN peptide and SHp peptide were purchased from ChinaPeptides (Shanghai, China). Melatonin and (4-Carboxybutyl)triphenylphosphonium bromide were purchased from Aladdin Bio-chem Technology (Shanghai, China). Maleimide-poly(ethylene glycol)2000-poly (lactic acid)5000 (Mal-PEG-PLGA) and methoxy poly (ethylene glycol)2000-poly(lactic acid)5000 (MePEG-PLGA) were purchased from Xi’an ruixi Biological Technology (Xi’an, China). JC-1 and DCFH-DA were purchased from Invitrogen (Carlsbad, USA). MTT was purchased from Solarbio technology (Beijing, China). Fetal bovine serum (FBS), DMEM media, PBS buffer, trypsin/EDTA, and penicillin–streptomycin were purchased from Hyclone Laboratories (Logan, USA). Superoxide dismutase (SOD) and Malonaldehyde (MDA) were purchased from Nanjing jiancheng Bioengineering Institute (Nanjing, China).
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3

Biochemical Markers in D-Galactose-Induced Aging

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All the kits including liver glycogen (LG), blood lactate acid (Fernández‐Tomé et al.), blood urea nitrogen (BUN), superoxide dismutase (SOD) (No.A‐005), malonaldehyde (MDA) (No.A003‐1), total cholesterol (TC) (No.A111‐1), glutathione peroxidase (GSH‐Px) (No.A005), catalase (CAT) (No.A007‐1), and total cholinesterase (AChE) (No.A024) were purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. D‐Galactose was obtained from Solarbio Technology Co. Ltd (Beijing, China). All other chemicals were of analytical grade.
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4

Measuring MDA and SOD Levels

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Malonaldehyde (MDA) and superoxide dismutase (SOD) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Levels of MDA and activity of SOD were measured by following the commercial kits’ protocols.
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