Fc receptor blocking was performed (clone 93; BioLegend) in FACS buffer (0.5% BSA, 2 mM EDTA, and 0.02% sodium azide in PBS) for 5 min at 4°C before surface staining (4°C for 30 min). The following anti-mouse antibodies from BioLegend were used for surface staining: APC/Cyanine7 anti-CD11b, PE/Cyanine7 anti-mouse CD19 Antibody, Brilliant Violet 711 anti-CD45, Alexa Fluor 647 anti-mouse CD206 (MMR) Antibody, Brilliant Violet 421 anti-mouse F4/80 Antibody, PE anti-mouse FcεRIα, Alexa Fluor 700 anti-mouse Ly-6C Antibody, Alexa Fluor 488 anti-mouse Ly-6G Antibody, Brilliant Violet 510 anti-mouse I-A/I-E Antibody, and PE/Cyanine7 anti-mouse Kit. The APC Rat Anti-Mouse CD19 was from BD Pharmingen. Live cells were distinguished by LIVE/DEAD Fixable Yellow Dead Cell Stain Kit (Thermo Fisher Scientific). Flow cytometry was performed on BD LSRFortessa X-20 flow cytometer. Flow cytometry data were analyzed with FlowJo software (Tree Star).
Apc rat anti mouse cd19
Manufactured by BD
The APC Rat Anti-Mouse CD19 is a laboratory reagent used for the identification and analysis of mouse CD19-positive cells. It is a flow cytometry antibody that binds to the CD19 antigen, which is expressed on the surface of B lymphocytes. This product is intended for research use only and its performance characteristics have not been established for diagnostic or clinical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Brilliant violet 421, by BioLegend (1 mentions)
Brilliant violet 711 anti cd45, by BioLegend (1 mentions)
Brilliant violet 510, by BioLegend (1 mentions)
Apc cyanine7 anti cd11b, by BioLegend (1 mentions)
Anti mouse antibodies, by BioLegend (1 mentions)
Live dead fixable yellow dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
Lsrfortessa x 20 flow cytometer, by BD (1 mentions)
Red blood cell lysis buffer, by Thermo Fisher Scientific (1 mentions)
Pe rat anti mouse cd11b, by BD (1 mentions)
Mouse fc block, by BD (1 mentions)
Bv510 hamster anti mouse cd3e, by BD (1 mentions)
Apc cy7 rat anti mouse cd45, by BD (1 mentions)
Pe cy7 rat anti mouse cd8a, by BD (1 mentions)
Percp cy5.5 rat anti mouse cd4, by BD (1 mentions)
Triple pure high impact zirconium 1.5 mm beads, by Benchmark Scientific (1 mentions)
Pe rat anti mouse cd41, by BD (1 mentions)
Cytometric bead array mouse inflammation kit, by BD (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using apc rat anti mouse cd19
1
Comprehensive Immune Cell Profiling
2
Immunology Protocol: Characterizing Murine Leukocytes
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The following reagents were used in this study: wild-type smooth strain Escherichia coli (E. coli) (O55:B5, ATCC® 12014™), LPS from E. coli (0111:B4) and RIPA buffer with protease inhibitor cocktail were from Sigma-Aldrich, St Louis, Missouri), pyrogen-free saline (Thermo Fisher Scientific, North Ryde, New South Wales, Australia), Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Biosciences, North Ryde, New South Wales, Australia), bovine serum albumin (BSA) (Sigma-Aldrich), alkaline phosphatase (AP) conjugated anti-mouse (Sigma-Aldrich), anti-rabbit and anti-human IgG (Sigma-Aldrich), β2GPI (Hematologic Technologies, Essex Junction, Vermont), isotype control rabbit polyclonal IgG (BD Biosciences) and red blood cell lysis buffer (eBioscience, San Diego, California). Triple-Pure High Impact Zirconium 1.5 mm beads (Benchmark Scientific, NJ) Affinity purified murine IgG2, anti-β2GPI monoclonal antibody and affinity purified polyclonal rabbit anti-β2GPI antibody were produced as previously described22 (link), 23 .
PE-rat anti-mouse CD41, APC-Cy7 rat anti-mouse CD45, BV510 hamster anti-mouse CD3e, PerCP-Cy5.5 rat anti-mouse CD4, PE-Cy7 rat anti-mouse CD8a, APC rat anti-mouse CD19, V450 rat anti-mouse LY-6G and LY-6C, PE rat anti-mouse CD11b and mouse Fc blockTM were purchased from BD Biosciences and used at 0.2 mg/mL.
PE-rat anti-mouse CD41, APC-Cy7 rat anti-mouse CD45, BV510 hamster anti-mouse CD3e, PerCP-Cy5.5 rat anti-mouse CD4, PE-Cy7 rat anti-mouse CD8a, APC rat anti-mouse CD19, V450 rat anti-mouse LY-6G and LY-6C, PE rat anti-mouse CD11b and mouse Fc blockTM were purchased from BD Biosciences and used at 0.2 mg/mL.
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