Collagen conjugation efficiency was determined by fluorescamine assay. Each hydrogel (containing 50 µL of neutralized collagen) was mixed with 25 µL of 3 mg/mL fluorescamine (Sigma-Aldrich; F9015) in DMSO. After a 70-minute incubation at room temperature with foil wrap to prevent light exposure, fluorescence intensity (Ex/Em = 380/470 nm) was measured using a SpectraMax M Series Multi-Mode Microplate Reader (Sunnyvale, CA). Using the linear standard curve generated by neutralized collagen from 0 to 3 mg/mL, conjugation efficiency was evaluated.
F9015
Manufactured by Merck Group
F9015 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use. The core function of F9015 is to perform specific tasks required in a laboratory setting. No further details about its intended use or capabilities can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700 confocal microscope, by Zeiss (2 mentions)
F7250, by Merck Group (1 mentions)
4 4 6 dimethoxy 1 3 5 triazin 2 yl 4 methyl morpholinium chloride, by Fujifilm (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Dulbecco s pbs, by Thermo Fisher Scientific (1 mentions)
Reagentplus, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Hexylamine, by Merck Group (1 mentions)
3 protocols using f9015
1
Collagen Conjugation Efficiency Analysis
2
Protein and Starch Visualization in Noodles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The morphology of protein/starch matrices (wheat flours and the inner and outer section of cooked noodles) was observed under an LSM 700 confocal microscope (Carl Zeiss, Germany) as described previously (Bhattarai et al., 2016) . Fluorescamine (F9015, Sigma) and fluorescein isothiocyanate (FITC; F7250, Sigma) were used for labelling protein and starch, respectively.
3
Cationic Chitosan Derivative-Based Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CTS (average MW, 370 kDa;
deacetylation degree 85%), succinyl-β-CD, trifluoroacetic acid
(TFA, ReagentPlus, 99%), fluorescamine (F9015, ≥98%), hexylamine
(99%), and dexamethasone [DEX, ≥98% (HPLC), powder] were purchased
from Sigma-Aldrich (St. Louis, MO). 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl
morpholinium chloride (DMTMM) was obtained from Wako Pure Chemicals
(Osaka, Japan). Ethyl alcohol anhydrous (EtOH, 99.9%) and methyl alcohol
(MeOH) were purchased from DaeJung (Chemical & Metals Co. Ltd.,
Korea). DCM (Extra Pure, 99.0%+) was purchased from Junsei (Junsei
Chemical Co. Ltd, Japan). Sodium hydroxide (NaOH) was purchased from
YPC (Yakuri Pure Chemicals CO., Ltd. Japan). Fibroblast NIH3T3 (CRL-1658)
was obtained from ATCC. DMEM, FBS, trypsin–EDTA, antibiotics
(PS, penicillin–streptomycin), and Dulbecco’s PBS were
purchased from Gibco (Rockville, MD, USA). Deionized-distilled water
(DW) was produced by an ultrapure water system (Puris-Ro800; Bio Lab
Tech., Korea). The dialysis membrane tube was purchased from Spectrum
Spectra (Spectra/Por 4 dialysis tubing, 12–14 K MWCO, 45 mm
flat width, 100 ft length). All other reagents and solvents were of
analytical grade and used without further purification.
deacetylation degree 85%), succinyl-β-CD, trifluoroacetic acid
(TFA, ReagentPlus, 99%), fluorescamine (F9015, ≥98%), hexylamine
(99%), and dexamethasone [DEX, ≥98% (HPLC), powder] were purchased
from Sigma-Aldrich (St. Louis, MO). 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl
morpholinium chloride (DMTMM) was obtained from Wako Pure Chemicals
(Osaka, Japan). Ethyl alcohol anhydrous (EtOH, 99.9%) and methyl alcohol
(MeOH) were purchased from DaeJung (Chemical & Metals Co. Ltd.,
Korea). DCM (Extra Pure, 99.0%+) was purchased from Junsei (Junsei
Chemical Co. Ltd, Japan). Sodium hydroxide (NaOH) was purchased from
YPC (Yakuri Pure Chemicals CO., Ltd. Japan). Fibroblast NIH3T3 (CRL-1658)
was obtained from ATCC. DMEM, FBS, trypsin–EDTA, antibiotics
(PS, penicillin–streptomycin), and Dulbecco’s PBS were
purchased from Gibco (Rockville, MD, USA). Deionized-distilled water
(DW) was produced by an ultrapure water system (Puris-Ro800; Bio Lab
Tech., Korea). The dialysis membrane tube was purchased from Spectrum
Spectra (Spectra/Por 4 dialysis tubing, 12–14 K MWCO, 45 mm
flat width, 100 ft length). All other reagents and solvents were of
analytical grade and used without further purification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!