Plate count agar media

The pour plate method was used to determine total viable count (TVC) and total psychrotrophic count (TPC). Ten grams of the fish minced sample was aseptically taken and homogenized in 90 mL of sterile 85% NaCl solution with a blender (HBM-400B, HBM Biomed, Tianjin, China) at room temperature. Appropriate dilutions were serially prepared and then 1 mL of each was spread onto plate count agar media (Merck, Darmstadt, Germany). The prepared plates were incubated at 37°C for 2 days for TVC, and at 10°C for 7 days for TPC. All counts were expressed as log colony-forming units (CFU)/g and performed in triplicate.

Total plate count (TPC) is one of the methods to estimate the total number of microorganisms in minced chicken. The TPC was determined using the pour plate method. Briefly, 10 g of the minced chicken sample was homogenized in 90 mL of sterile 85% NaCl solution with a blender (HBM-400B, HBM Biomed, Tianjin, China) at room temperature. An appropriate dilution was serially prepared. Then, 1 mL of each dilution was spread onto plate count agar media (Merck, Germany). The prepared plates were incubated at 37 °C for 7 days for TPC. Thus, all counts were expressed as log colony-forming unit (CFU)/g, and all measurement was conducted in triplicate.