Avance 400 mhz instrument

¹H, ¹³C and 2D NMR spectra of the purified transglycosylated products were recorded on a Brucker Avance 400 MHz instrument at 25 °C, using TMS as an internal standard.

2-Acetylpyridine (≥99%), 2-acetylthiazole (99%), thiosemicarbazide (99%) and Girard’s T reagent (99%) were obtained from Aldrich. IR spectra were recorded on a Nicolet 6700 Fourier transform infrared (FT-IR) spectrometer using the attenuated total reflectance (ATR) technique in the region 4000−400 cm⁻¹ (vs-very strong, s-strong, m-medium, w-weak, bs-broad signal). ¹H- and ¹³C-NMR spectra were recorded on a Bruker Avance Ultrashield 500 plus spectrometer (¹H at 500 MHz; ¹³C at 125 MHz) at room temperature using TMS as internal standard in DMSO-d₆, or on a Varian Mercury 200 MHz spectrometer (¹H at 200 MHz; ¹³C at 50 MHz) or a Brucker Avance 400 MHz instrument (¹H at 400 MHz; ¹³C at 101 MHz) using CDCl₃ as the solvent. Chemical shifts are expressed in ppm (δ) values and coupling constants (J) in Hz. Elemental analyses (C, H, N and S) were performed by standard micro-methods using the ELEMENTARVario ELIII C.H.N.S.O analyzer. Molar conductivities were measured at room temperature (25 °C) on a digital conductivity-meter JENWAY-4009. GC/MS spectra were recorded with a Shimandzu R GCMS-QP2010 Plus Chromatograph Mass Spectrometer using a MEGAR (MEGA-5, F.T: 0.25 μm, I.D.: 0.25 mm, L: 30 m, Tmax: 350 °C, Column ID# 11475) column, using n-octane as the internal standard.