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Pullulan gel filtration standards

Manufactured by Polymer Laboratories
Sourced in United Kingdom

Pullulan gel filtration standards are a set of reference materials used to calibrate and verify the performance of gel filtration chromatography systems. These standards are composed of pullulan, a naturally occurring polysaccharide, with a well-defined range of molecular weights. They are designed to ensure the accurate measurement of the molecular weight distribution of samples during gel filtration analysis.

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2 protocols using pullulan gel filtration standards

1

Alginate Hydrogel Viability Assay

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OligoG CF-5/20 and the high molecular weight alginate PRONOVA UP MVG (>60% guluronic acid and Mw of 200,000 g/mol) were provided by AlgiPharma AS (Sandvika, Norway). The LIVE/DEAD® BaclightTM Bacterial Viability kit was from Invitrogen Molecular Probes (Paisley, UK). Pullulan gel filtration standards were from Polymer Laboratories (Church Stretton, UK). All chemicals were obtained from either Fisher Scientific (Loughborough, UK) or Sigma-Aldrich (Poole, UK) unless otherwise stated and were of analytical grade.
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2

Characterization of Type I Dextrin

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Type I dextrin from corn
(Mw = 7500 g/mol), colistin sulfate, α-amylase
from human saliva, N-hydroxysulfosuccinimide (sulfo-NHS),
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT),
bicinchoninic acid (BCA) solution, and dimethyl sulfoxide (DMSO) maltose
oligomers [degree of polymerization (DP) 2, 3, 6, and 7] were purchased
from Sigma-Aldrich (Poole, U.K.). 1-Ethyl-3-(3-(dimethylamino)propyl
carbodiimide hydrochloride) (EDC) was acquired from Pierce (Rockford,
USA). Disodium hydrogen phosphate, potassium dihydrogen phosphate,
potassium chloride, 4-dimethylaminopyridine, and sodium chloride were
from Fisher Scientific (Loughborough, U.K.). Pullulan gel filtration
standards (Mw = 11 800–210 000
g/mol) were purchased from Polymer Laboratories (Church Stretton,
U.K.). Unless otherwise stated, all chemicals were of analytical grade
and used as received. All solvents were of general reagent grade (unless
stated) and were from Fisher Scientific (Loughborough, U.K.).
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