For the inhibition of CD39, freshly isolated cells were firstly preincubated with 10 μM sodium metatungstate (POM‐1) (Selleck) for 2 h. Next, 100 μM ATP (Selleck) was added to the culture system, and the cells were incubated for 24 h. For the inhibition of CD73, freshly isolated cells were firstly preincubated with 1 μM LY‐3475070 (Selleck) for 2 h. Next, 100 μM adenosine monophosphate (AMP) (Selleck) was added to the culture system, and the cells were incubated for 24 h. For the inhibition of A2AR, freshly isolated cells were firstly preincubated with ZM241385 (1 μM) (TargetMol) or Istradefylline (1 μM) (Selleck) for 2 h. Next, 5 μM ADO (Selleck) was added to the culture system, and the cells were incubated for 24 h. A total of 1 million cells were added to each well. When it is difficult to meet the cell number requirement, we would make the number of cells in the experimental group as the same as that in the control group.
Pom 1
Manufactured by Selleck Chemicals
The POM-1 is a laboratory instrument designed for the measurement and analysis of various physical and chemical properties. It is a versatile piece of equipment that can be used in a wide range of scientific and industrial applications. The core function of the POM-1 is to provide accurate and reliable data through its advanced measurement capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pom 1
1
Inhibition of CD39, CD73, and A2AR
2
Macrophage-NSCLC Cell Crosstalk
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Healthy donor-derived macrophages (5 × 105/well) and NSCLC cells (H460/A549, 8 × 105/well) were co-cultured in a Transwell device (0.4um). CPI-444 (Selleck, S6646), sodium metatungstate (POM-1) (Selleck, S5525), and JSH-23 (Selleck, S7351) were added 2 h before 24 h incubation. The controls included individual cultures at matching densities. Supernatants and cells were collected for protein and mRNA analysis.
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