Gentamycin sulfate solution

Wool samples from Akkaraman sheep breed (Ovis aries Linnaeus, 1758) localized in the Central Anatolian region of Turkey were kindly provided by Lalahan Livestock Central Research Institute (Ankara, Turkey) [35] . Acetic acid, chloroform, ethanol, hydrochloric acid (HCl), L-glutamine, 2-(N-morpholino)ethanesulfonic acid (MES), sodium dodecyl sulfate (SDS) and tris-base were purchased from Merck (Darmstadt, Germany). Methanol, tris-HCl, perAcetic acid solution (32 w % in dilute Acetic acid), sodium chloride, sodium hydroxide, sodium phosphate monobasic, Fourier transform infrared (FTIR) spectroscopy grade potassium bromide (KBr), TruPAGE® precast gels (10 × 8 cm with 4-12% gradient), cellulose membrane dialysis tubing (43 × 27 mm, MWCO ~14,000 Da), and CCK-8 cell counting kit were obtained from Sigma-Aldrich (Sigma-Aldrich Co., St. Louis, MO, USA). High glucose DMEM cell culture medium, European grade fetal bovine serum (FBS), trypsin-EDTA 0.25% solution, trypan blue solution (5 mg/ml), and gentamycin sulfate solution (50 mg/ml) from Biological Industries (Cromwell, CT, USA) were used in the cell proliferation experiments. Coomassie brilliant blue R-250 and PageRulerTM pre-stained protein ladder were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All reagents were used without any purification.