Ri 1.514 objective immersion oil

For wide-field imaging, living SCs were imaged using a DeltaVision Elite wide-field fluorescence deconvolution microscope (GE Healthcare Life Sciences) equipped with a ×100, NA 1.4 UPlanSApo oil objective lens (Olympus), and a Cool SNAP HQ2 CCD camera (Photometrics). The images acquired were typically z-stacks spanning 8–12 µm depth with a z-distance of 0.2 µm. Images were subsequently deconvolved using the Resolve 3D-constrained iterative deconvolution algorithm within the softWoRx 5.5 software package (GE Healthcare Life Sciences).
Confocal images of accessory glands were acquired by using LSM 880 laser scanning confocal microscope equipped with a ×63, NA 1.4 Plan APO oil DIC objective (Carl Zeiss). RI 1.514 objective immersion oil (Carl Zeiss) was employed.

Confocal images of cultured cells and AGs were acquired either by using a Zeiss LSM 510 Meta [Axioplan 2], an Olympus FV1000 microscope set‐up with 100× NA1.4 oil objective or an LSM 880 laser scanning confocal microscope equipped with a 63×, NA 1.4 Plan APO oil DIC objective (Carl Zeiss). RI 1.514 objective immersion oil (Carl Zeiss) was employed.