Camptothecin (CPT), etoposide (Eto), 4-OH tamoxifen (Tam), doxycycline (Dox), triptolide (Tript), and auxin were purchased from Sigma. auxinole was from MedChemexpress (HY-111444), puromycin (A11138–03) and hygromycin B (10687010) were purchased from Thermo Fisher. MG132 was purchased from Cayman Chemical. Mouse monoclonal anti-TOP1 (C-21), anti-c-MYC (9E10), anti-MAX (H-2), anti-TOP2A (E-10), anti-N-MYC (B8.4.B), anti-nucleolin (C-23) and anti-IgG [mouse (Sc-2025), rabbit (Sc-2027)] antibodies were from Santa Cruz Biotechnology. Rabbit monoclonal anti-c-MYC antibody (N-ter) (Y69) (ab32072), anti-TOP1 (N-ter) (ab109374), anti-TOP2A (ab52934) and mouse monoclonal anti-c-MYC (C-ter) (ab56), anti-GAPDH (ab9484), anti-RNAPII (ab5408) and anti-Histone H3 (ab1791) were from Abcam. Rabbit polyclonal anti-c-MYC (mid-ter) was from Novus (NBP2–49201). Anti-FLAG antibody (F1804) was from Sigma. Anti-KAP1 antibody (A300–274A) was from Bethyl. Spike-in antibody (61686) was purchased from Active motif. Rabbit polyclonal anti-GFP (A-11122) was from Thermo Fisher. Rabbit polyclonal anti-TOP2B antibody (20549–1-AP) was from Proteintech (Rosemont, IL, USA). Secondary antibodies like horseradish peroxidase conjugated anti-rabbit IgG (ab205718) or anti-mouse IgG (ab205719) were purchased from Abcam.
Spike in antibody 61686
Manufactured by Active Motif
The Spike-in antibody (61686) is a laboratory tool designed to assist in the analysis and quantification of protein samples. Its core function is to provide a known, consistent amount of a specific antibody that can be used as a reference or control in various experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
4 oh tamoxifen, by Merck Group (1 mentions)
Anti flag antibody f1804, by Merck Group (1 mentions)
Hygromycin b, by Thermo Fisher Scientific (1 mentions)
Etoposide, by Merck Group (1 mentions)
Doxycycline, by Merck Group (1 mentions)
Camptothecin, by Merck Group (1 mentions)
Auxin, by Merck Group (1 mentions)
Triptolide, by Merck Group (1 mentions)
Puromycin a11138 03, by Thermo Fisher Scientific (1 mentions)
Anti histone h3 ab1791, by Abcam (1 mentions)
Mg132, by Cayman Chemical (1 mentions)
Chip it high sensitivity kit, by Active Motif (1 mentions)
Tissueruptor, by Qiagen (1 mentions)
Epishear probe sonicator, by Active Motif (1 mentions)
2 protocols using spike in antibody 61686
1
Comprehensive Reagents Inventory for Cell Biology
2
Chromatin Immunoprecipitation of H3K27Ac and GR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ChIP was performed using the ChIP-IT High Sensitivity kit (Active Motif) according to manufacturer’s instructions. A TissueRuptor (QIAGEN) was used for 45 seconds at maximum speed to disrupt liver tissue, followed by Dounce homogenizing for 70 strokes to release cell nuclei. Nuclear sonication was conducted with an EpiShear Probe Sonicator (Active Motif, 3.5-mm probe) for 8 rounds of 2 minutes per sample, at 37% amplitude. 15 μg sheared chromatin was incubated with anti-H3K27Ac antibody (Active Motif, lot 31814008) or an anti-GR antibody cocktail (Proteintech 24050-1-A, lot 00044414, and Cell Signaling Technology, D8H2, lot 2). For GR ChIP, 30 ng spike-in Drosophila melanogaster chromatin (Active Motif) plus an antibody to the Drosophila-specific histone variant H2Av (Active Motif Spike-in Antibody 61686; lot 34216004) (44 (link)) were include in the IP reaction. Antibody was pulled down with magnetic protein G agarose microspheres (ReSyn Biosciences). After elution and de-crosslinking, ChIP DNA was purified, as per the kit instructions.
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