The surface markers of RMDMs were quantified by flow cytometry as described previously [14 ]. First, the RMDMs were stained with anti-CD206-MR5D3 (Novus Biologicals, USA) and anti-CD80-PE (Thermo Fisher Scientific, USA). After fixing, the RMDMs were subjected to flow cytometric analysis (Accuri C6; Becton Dickinson, USA). FlowJo software (Tree Star Inc., USA) was used for data analysis. The levels of TGF-β, IFN-γ, IL-4 and −12 in RMDMs from HSP and normal rats were determined using the corresponding ELISA kits (R&D Systems, USA) as per the manufacturer’s protocols.
Anti cd206 mr5d3
Manufactured by Novus Biologicals
Sourced in United States
Anti-CD206-MR5D3 is a mouse monoclonal antibody that recognizes the human CD206 (mannose receptor) protein. CD206 is a cell surface receptor expressed on the surface of macrophages and dendritic cells and is involved in the endocytosis of glycoproteins.
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2 protocols using anti cd206 mr5d3
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Characterization of RMDM Surface Markers
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Characterization of RMDM Surface Markers
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The surface markers of RMDMs were quantified by flow cytometry as described previously [14 ]. First, the RMDMs were stained with anti-CD206-MR5D3 (Novus Biologicals, USA) and anti-CD80-PE (Thermo Fisher Scientific, USA). After fixing, the RMDMs were subjected to flow cytometric analysis (Accuri C6; Becton Dickinson, USA). FlowJo software (Tree Star Inc., USA) was used for data analysis. The levels of TGF-β, IFN-γ, IL-4 and −12 in RMDMs from HSP and normal rats were determined using the corresponding ELISA kits (R&D Systems, USA) as per the manufacturer’s protocols.
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