The full-length ORF of clusterin, HSP90 were amplified from total cDNA of GCSC using high-fidelity AccuPrime Taq polymerase (Life Technology), and cloned into the MluI and EcoRI sites of pLVX-TetOne-Puro inducible expression vector (Clontech) using In-Fusion HD Cloning Kit (Clontech) following the manufacturer's instructions. A FLAG tag (DYKDDDDK) was added to the C terminal of Clusterin protein. pLVX-TetOne-Puro inducible expression vector (Clontech) was used as control.
Plvx tetone puro inducible expression vector
Manufactured by Takara Bio
The PLVX-TetOne-Puro is an inducible expression vector that allows for the controlled expression of genes of interest. It contains a tetracycline-responsive promoter that enables the regulation of transgene expression in response to the presence or absence of tetracycline or its derivatives.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using plvx tetone puro inducible expression vector
1
Inducible Expression of Clusterin and HSP90
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